Anti-ADAM17 (mouse), Pro Domain Antibody
This rabbit IgG polyclonal antibody was generated against the pro-domain of mouse ADAM17 and is specific for the pro-domain of mouse ADAM17.
The ADAM (A Disintegrin And Metalloprotease Domain) family is a unique group of proteins that has the ability to cleave or shed extracellular portions of transmembrane proteins. In addition, ADAMs are involved in cell-cell and cell-matrix interactions (e.g., fertilization, muscle development and neurogenesis).
ADAM17 (also referred to as Tumor Necrosis Factor Convertase, TACE) is involved in the processing of tumor necrosis factor alpha (TNF-a) at the surface of the cell, and is also required for shedding several ligands of the epidermal growth factor receptor and many other membrane proteins. The prodomain of catalytically active ADAMs is thought to act as an inhibitor of the protease via a cysteine-switch mechanism. Prodomain removal is therefore thought to be a prerequisite for ADAM17 protease activity.
From the laboratory of Carl P. Blobel, MD, PhD, Hospital for Special Surgery.
Part of The Investigator's Annexe program.
This rabbit IgG polyclonal antibody was generated against the pro-domain of mouse ADAM17 and is specific for the pro-domain of mouse ADAM17.
The ADAM (A Disintegrin And Metalloprotease Domain) family is a unique group of proteins that has the ability to cleave or shed extracellular portions of transmembrane proteins. In addition, ADAMs are involved in cell-cell and cell-matrix interactions (e.g., fertilization, muscle development and neurogenesis).
ADAM17 (also referred to as Tumor Necrosis Factor Convertase, TACE) is involved in the processing of tumor necrosis factor alpha (TNF-a) at the surface of the cell, and is also required for shedding several ligands of the epidermal growth factor receptor and many other membrane proteins. The prodomain of catalytically active ADAMs is thought to act as an inhibitor of the protease via a cysteine-switch mechanism. Prodomain removal is therefore thought to be a prerequisite for ADAM17 protease activity.
From the laboratory of Carl P. Blobel, MD, PhD, Hospital for Special Surgery.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | ADAM17 Pro-Domain |
| Accession ID: | P78536 |
| Molecular Weight: | ~120 kDa |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Mouse |
| Immunogen: | Pro-domain of mouse ADAM17 |
| Species Immunized: | Rabbit |
| Epitope: | Pro-domain |
| Purification Method: | Serum, not purified |
| Tested Applications: | WB (1:250) |
| Storage: | -80C |
| Shipped: | Dry ice |
| Antibody | Species Reactivity | Specificity |
| ADAM9 | Human | Cytoplasmic Domain |
| ADAM9 | Mouse | Cytoplasmic Domain |
| ADAM12 | Mouse | Cytoplasmic Domain |
| ADAM15 | Human | Cytoplasmic Domain |
| ADAM15 | Mouse | Cytoplasmic Domain |
| ADAM15 | Human | Extracellular Domain |
| ADAM15 | Mouse | Disintegrin Domain |
| ADAM17 | Mouse | Cytoplasmic Domain |
| ADAM17 | Mouse | Pro domain |
| ADAM19 | Mouse | Cytoplasmic Domain |
| ADAM28 | Mouse | Cytoplasmic Domain |
| ADAM28 | Mouse | Extracellular Domain |
| SNX9 | Mouse | Cytoplasmic Domain |
Western Blot Analysis
Antibodies against: pro-domain fused to GST (α-PRO), cytoplasmic domain fused to GST (α-CYTO).
Adapted from: Schlöndorff J, et al. JBiochem J. 2000, 347, 131-138.
- Schlöndorff, J.; Becherer, J. D.; Blobel, C. P. Intracellular maturation and localization of the tumour necrosis factor alpha convertase (TACE). Biochem J. 2000, 347, 131-138.
- McIlwain DR, Lang PA, Maretzky T, Hamada K, Ohishi K, Maney SK, Berger T, Murthy A, Duncan G, Xu HC, Lang KS, Häussinger D, Wakeham A, Itie-Youten A, Khokha R, Ohashi PS, Blobel CP, Mak TW. iRhom2 regulation of TACE controls TNF-mediated protection against Listeria and responses to LPS. Science. 2012 Jan 13;335(6065):229-32.
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