Custom Lentivirus Production
Our custom lentivirus product service provides customers with maximal flexibility and top quality. Our services range from construct design, to lentivral vector production, concentration and titration.
Lentiviral vectors are a powerful tool for delivering target genes into almost all types of dividing and non-dividing mammalian cells in vitro and in vivo. However, production of recombinant lentiviral vectors is laborious and challenging, especially when high titer of recombinant lentiviral vectors (>107 transducing unit (TU)/ml) are required.
Our standardized and optimized custom lentiviral vector production and strict quality control systems enables us to generate high titer of functional recombinant lentiviral vectors for our customers. Our titration procedures determine the quantity of functional lentiviral vectors rather than physical lentiviral particles.
Our custom lentivirus product service provides customers with maximal flexibility and top quality. Our services range from construct design, to lentivral vector production, concentration and titration.
Lentiviral vectors are a powerful tool for delivering target genes into almost all types of dividing and non-dividing mammalian cells in vitro and in vivo. However, production of recombinant lentiviral vectors is laborious and challenging, especially when high titer of recombinant lentiviral vectors (>107 transducing unit (TU)/ml) are required.
Our standardized and optimized custom lentiviral vector production and strict quality control systems enables us to generate high titer of functional recombinant lentiviral vectors for our customers. Our titration procedures determine the quantity of functional lentiviral vectors rather than physical lentiviral particles.
| Product Type: | Virus |
| Name: | Custom Lentivirus |
| Biosafety Level: | BSL-2 |
| Vector Information: | pLV-CE |
| Promoter: | Various available |
| Selection Gene: | Various available |
| Titer: | See service summary table |
| Storage: | Store at -80C. Multiple freeze/thaw cycles not recommended. |
| Shipped: | Dry ice |
Standard Plasmid Map from which Premade Lentiviruses are Generated
Premade Lentivirus Transduction Protocol
| Service Description |
Titer
|
Volume
|
Turnaround Time
|
Price*
|
| Custom Crude Lentivirus Production |
N/A
|
10 mL
|
2-3 Weeks
|
$350
|
| Custom Low-Titer of Lentivirus Production |
>107 TU/mL
|
1 mL
|
2-3 Weeks
|
$890
|
| Custom Medium-Titer of Lentivirus Production |
>108 TU/mL
|
200 µL
|
2-3 Weeks
|
$1490
|
| Custom High-Titer of Lentivirus Production |
>109 TU/mL
|
200 µL
|
2-3 Weeks
|
$2090
|
| Custom Ultra-High-Titer of Lentivirus Production |
>1010 TU/mL
|
20 µL
|
2-3 Weeks
|
$2490
|
| Custom-Titer and Scale of Lentivirus Production |
Custom
|
Custom
|
2-3 Weeks
|
How Our Custom Lentivirus Production Works
If your lentiviral construct is ready, please provide us:
- The construct map or sequence.
- 1-5 µg of your lentiviral construct plasmid DNA or bacterial culture.
We will prepare Endotoxin-free plasmid and transfect our packaging cell line for your lentivirus production. Harvest lentivirus particle followed by concentration and titration.
If you need us to generate lentiviral vector for your target gene, please:
- Choose lentiviral vector format from our vector collection and specify the gene of interest.
- Please send us 1-5 µg plasmid or bacterial culture for your gene if it is available, otherwise send us cDNA sequence.
We will subclone the gene of interest or shRNA into chosen lentiviral vector and confirm by sequencing, prepare high quality plasmid DNA and transfect packaging cell line, harvest lentivirus particle followed by concentration and titration.
Have Questions? Please contact us with your specific needs or any questions.
If you publish research with this product, please let us know so we can cite your paper.
