Anti-SNF2-Related -CBP-Activator Protein (SRCAP) Antibody (Affinity Purified)
This affinity purified antibody was raised in rabbits against the C-terminal SRCAP peptide (SSAPPSLAGPAVSHRGRKAKT) and is recommended for detection of human SRCAP. It has been tested for use in Chromatin immunoprecipitation assays (ChIP), IP, and WB studies.
The SRCAP (SNF2-Related-CBP-Activator Protein) gene encodes the core catalytic component of the multiprotein chromatin-remodeling SRCAP complex. The encoded protein is an ATPase that is necessary for the incorporation of the histone variant H2A.Z into nucleosomes. It can function as a transcriptional activator in Notch-mediated, CREB-mediated and steroid receptor-mediated transcription. Mutations in this gene cause Floating-Harbor syndrome, a rare disorder characterized by short stature, language deficits and dysmorphic facial features.
From the laboratory of John Chrivia, PhD, St. Louis University.
Part of The Investigator's Annexe program.
This affinity purified antibody was raised in rabbits against the C-terminal SRCAP peptide (SSAPPSLAGPAVSHRGRKAKT) and is recommended for detection of human SRCAP. It has been tested for use in Chromatin immunoprecipitation assays (ChIP), IP, and WB studies.
The SRCAP (SNF2-Related-CBP-Activator Protein) gene encodes the core catalytic component of the multiprotein chromatin-remodeling SRCAP complex. The encoded protein is an ATPase that is necessary for the incorporation of the histone variant H2A.Z into nucleosomes. It can function as a transcriptional activator in Notch-mediated, CREB-mediated and steroid receptor-mediated transcription. Mutations in this gene cause Floating-Harbor syndrome, a rare disorder characterized by short stature, language deficits and dysmorphic facial features.
From the laboratory of John Chrivia, PhD, St. Louis University.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | SRCAP |
| Accession ID: | Q6ZRS2, NP_006653 |
| Molecular Weight: | 400 kDa |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Clone Name: | SLU1 |
| Reactivity: | Human (use with other species has not been tested) |
| Immunogen: | Peptide |
| Species Immunized: | Rabbit |
| Epitope: | C-terminal SCRAP peptide (SSAPPSLAGPAVSHRGRKAKT) |
| Purification Method: | Affinity purified using anti-peptide column |
| Buffer: | PBS |
| Tested Applications: | IP (1:100), WB (1:1000), and ChIP assay (1:100) |
| Concentration: | 1 mg/mL, (Bradford assay) |
| Storage: | -80C |
| Shipped: | Cold packs |
Demonstration of WB and IP analyses.
SRCAP protein was immunoprecipitated from AA549 Nuclear extracts with (SLU1) or normal rabbit IgG and analyzed by Western blot using anti-SRCAP antibody (SLU2).
- The chromatin remodeling protein, SRCAP, is critical for deposition of the histone variant H2A.Z at promoters. (2007). Wong, M.M., Cox L.K. and Chrivia, J.C. (2007). . J Biol Chem. 282, 26132-26139.
- Purification of a Human SRCAP Complex that Remodels Chromatin by Incorporating the Histone Variant H2A.Z into Nucleosomes. (2006). Ruhl, D.D., Jin, J. Cai, Y. Swanson, S., Florens, L., Michael P. Washburn, M.P., Conaway, R. and Conaway, J.W. and Chrivia, J.C.. Biochemistry 45, 5671-5677.
- Greenberg RS, Long HK, Swigut T, Wysocka J. Single Amino Acid Change Underlies Distinct Roles of H2A.Z Subtypes in Human Syndrome. Cell. 2019 Sep 5;178(6):1421-1436.e24. View article
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