Anti-phospho Charged Multivesicular Body Protein 4c, Ser210/214/215 [pCHMP4C] Antibody

This rabbit IgG1 polyclonal antiobody was generated against a synthetic peptide (TARRSRAASSQRAEEC) and recognizes mammalian forms of Phospho Charged multi vesicular protein 4c.

Borealin interacts directly with Snf7/Shrb/CHMP4 components in both Drosophila and human cells and the two proteins colocalize at the midbody in late cytokinesis. Aurora B phosphorylates CHMP4C at three serine residues located in its C-terminal linker region, a part of the protein known to regulate its ability to form polymers and interact with the membrane. Over-expression of CHMP4C variants mutated in these three residues caused cytokinesis failure, suggesting that Aurora B inhibits CHMP4C activity during cytokinesis. It is proposed that CPC controls abscission timing in both flies and human cells by regulating the function of ESCRT-III Snf7 proteins during cytokinesis through the interaction of its Borealin component with the N-terminus of Shrb/CHMP4 proteins and Aurora B-mediated phosphorylation of the CHMP4C regulatory linker tail.

From a laboratory at Cancer Research Technology.

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Catalog Number Product Size AVAILABILITY Price Qty
EW2003
Anti-phospho Charged Multivesicular Body Protein 4c, Ser210/214/215 [pCHMP4C] Antibody
100uL In stock
Regular Price:$350.00
Specifications

Product Type: Antibody
Antigen: Phospho Charged multi vesicular protein 4c
Molecular Weight: Approx. 30kDa
Isotype: IgG1
Clone Name: pCHMP4C
Reactivity: Mammalian
Immunogen: Synthetic peptide (TARRSRAASSQRAEEC)
Buffer: PBS, 0.09% NaN3
Positive Control: Synchronised HeLa cell extracts. Signals are absent in a twin blot that had been pre-incubated with lambda-phosphatase, indicating that the antibody specifically recognizes a phosphorylated form of CHMP4C.
Tested Applications: WB
Comments: Staining Pattern: CHMP4C is predominantly cytoplasmic and partially colocalizes with Borealin during late cytokinesis, and during abscission CHMP4 is found at the midbody.
Storage: -20C
Shipped: Cold Pack

Provider
From a laboratory at Cancer Research Technology.
References
  1. Capalbo et al. 2012. Open Biol. 2:120070 PMID: 22724069.

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