This mouse monoclonal antibody was generated against plant actin ACT11 and recognizes subclasses 2 and 3 of vegetative and subclasses 4 and 5 of pollen specific plant actins (arabidopsis ACT1, 3, 4, 7, 11, 12).
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
From the laboratory of Richard B. Meagher, PhD, University of Georgia.
Part of The Investigator's Annexe program.
|Molecular Weight:||45 kDa|
|Clone Name:||mAb2345a (2G-10)|
|Reactivity:||Plant actins, others not tested|
|Immunogen:||Purified Arabidopsisactin (ACT11)|
|Epitope:||Thr43 in plant actin, Thr41 in Dictyostelium|
|Purification Method:||Protein G column|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB, IF|
Western Blot Analysis
Immunofluorescence Staining Analysis
Adapted from: Kandasamy, M.K., et al. (2001) Plant Cell 13, 1541-54.
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