Anti-Serum Response Factor (SRF) Antibody
This polyclonal antibody was produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region (aa 400-450) of human Serum Response Factor (SRF) and is specific to mammalian and avian SRF.
Human Serum Response Factor (508 amino acids) is a member of the MADS box family of transcription factors. SRF regulates many genes involved in cell cycle control, differentiation, apoptosis, and embryonic development through interactions with the conserved promoter serum response element. The diverse functions of SRF are also mediated through the recruitment of many general and sequence-specific transcription factors, highlighting the central role of protein interaction in transcriptional regulation by SRF.
SRF itself can be activated by many agents including serum, cytokines, growth factors, tumor promoters, LPS, and UV light. The gene is a primary target of the MAPK signal transduction pathway through the ternary complex factors (TCFs). RhoA signaling also regulates the activity of SRF during muscle differentiation.
From the laboratory of Te-Chung Lee, PhD, University at Buffalo.
Part of The Investigator's Annexe program.
This polyclonal antibody was produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region (aa 400-450) of human Serum Response Factor (SRF) and is specific to mammalian and avian SRF.
Human Serum Response Factor (508 amino acids) is a member of the MADS box family of transcription factors. SRF regulates many genes involved in cell cycle control, differentiation, apoptosis, and embryonic development through interactions with the conserved promoter serum response element. The diverse functions of SRF are also mediated through the recruitment of many general and sequence-specific transcription factors, highlighting the central role of protein interaction in transcriptional regulation by SRF.
SRF itself can be activated by many agents including serum, cytokines, growth factors, tumor promoters, LPS, and UV light. The gene is a primary target of the MAPK signal transduction pathway through the ternary complex factors (TCFs). RhoA signaling also regulates the activity of SRF during muscle differentiation.
From the laboratory of Te-Chung Lee, PhD, University at Buffalo.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | Human Serum Response Factor (SRF) |
| Accession ID: | J03161 |
| Molecular Weight: | ~50kDa |
| Isotype: | Rabbit serum |
| Clonality: | Polyclonal |
| Reactivity: | Mammalian and avian |
| Immunogen: | Recombinant SRF protein |
| Species Immunized: | Rabbit |
| Tested Applications: | WB (1:1000), IF (1:1000) |
| Storage: | -20C or -80C |
| Shipped: | Cold packs |
Detection of SRF in C2C12 myoblasts
Cultured C2C12 myoblasts were fixed with methanol and stained with the SRF antibody (1:1000 dilution overnight at 4C). Green color denotes SRF staining, which is concentrated in nuclei.
- Walowitz JL, Bradley ME, Chen S, Lee T. Proteolytic regulation of the zinc finger transcription factor YY1, a repressor of muscle-restricted gene expression. J Biol Chem. 1998 Mar 20;273(12):6656-61.
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