This IgG2b mouse antibody was generated against amino acids 37-53 of rat GABA-A receptor gamma2 subunit (GABRG2) and is specific for the N-terminal extracellular domain, allowing for the detection of surface expressed receptors for live imaging.
Recombinant versions available at our sister company, Absolute Antibody:
GABA-A receptors are ligand-gated neurotransmitter receptors that function to mediate the fast synaptic inhibition within the brain. They are pentamers, composed from a variety of classes of subunits with gamma being just one class. Each subunit is composed of an extracellular N-terminal domain, four membrane spanning units, and a small extracellular C-terminal segment. Several mutations in the GABA(A) receptor subunit gamma-2 expression have been linked to epileptic syndromes that result in fibrile seizures.
From the laboratory of Jaideep Kapur, MD, PhD, University of Virginia.
|Antigen:||GABA-A Receptor Gamma 2 Subunit|
|Reactivity:||Reactive against the N-terminal extracellular domain (amino acids 37-53) of the gamma2 subunit of the GABA-A protein in rats|
|Immunogen:||Generated against the gamma2 subunit of the GABA-A protein from rats|
|Epitope:||N-terminal extracellular domain (amino acids 37-53)|
|Purification Method:||Protein G|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB, IF, IHC, IP|
Detection of g2 subunit in a Western blot assay using 10F10-C1-B8 antibody.
(A) 10, 25, 50, and 100 mg rat or mousehippocampal proteins were Western blotted using 2 mg/mL of 10F10-C1-B8 antibody. Arrowhead indicates g2 signal.Position of molecular weight marker 30, 40, and 50 kDa is also shown for reference (lane M). (B) Expression of g2 subunitin 10?100 mg rat or mouse hippocampal proteins in a Western blotting assay using 2 mg/mL rabbit polyclonal antibody.Arrowhead indicates g2 signal. Lane M shows position of marker proteins of 30, 40, and 50 kDa sizes. (C) Expression of ratg2L subunit in HEK293 cells (lanes T) transfected with a cDNA expressing rat g2L subunit. 75 mg total proteins were Westernblotted using 10F10-C1-B8 antibody (2 mg/mL, panel Ms) or rabbit polyclonal antibody (2 mg/mL, panel Rb). Proteins fromuntransfected cells were used as control (lanes NT). Position of molecular weight proteins 40 and 50 kDa is also shown(lane M). (D) g2 subunit was immunoprecipitated from 500 mg hippocampal proteins using 100 mg of 10F10-C1-B8 antibody(lane Ms), a rabbit polyclonal antibody against g2 subunit (lane Rb), or normal mouse IgG (lane MsIgG). Arrowhead indicatesg2 signal. In lane M, molecular weight marker, 40 and 50 kDa is marked. Adapted from: Joshi S, Sun C, Kapur J. A mouse monoclonal antibody against the gamma2 subunit of GABA-A receptors. Hybridoma. 30(6): 537-42 (2011).
Immunohistochemical detection of g2 subunit using 10F10-C1-B8 antibody.
(A) Expression of g2 subunit (red) inHEK293 cells transfected with rat g2L subunit cDNA. (B) Immunoreactivity of 10F10-C1-B8 antibody in untransfectedHEK293 cells. (C) Immunoreactivity of pre-adsorbed 10F10-C1-B8 antibody (1 mg/mL) in transfected HEK293 cells.(D) Immunoreactivity (red) of 10F10-C1-B8 in HEK293 cells transfected with 1 mg g2L cDNA and 5 mg anti-g2 subunit siRNA(green). (E, F) Expression of g2 subunit (red) in cultured hippocampal neurons grown in vitro for 12 days using 10F10-C1-B8antibody before and after pre-adsorption, respectively. Expression of MAP2 (blue) is also shown. (G) Expression of g2 subunit(red) in cultured hippocampal neurons grown in vitro for 6 days, and transfected with 5 mg of anti-g2 subunit siRNA (green)detected using 10F10-C1-B8 antibody. Expression of MAP2 (blue) is also shown. (H) Immunohistochemical detection ofg2 subunit in a control neuron not expressing siRNA and grown in vitro for 8 days. Adapted from: Joshi S, Sun C, Kapur J. A mouse monoclonal antibody against the gamma2 subunit of GABA-A receptors. Hybridoma. 30(6): 537-42 (2011).
For western blot, 1:2,000 dilution recommended.
For IHC, 1:250 dilution recommeneded.
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