Anti-Adenylate Cyclase Toxin [3D1] Antibody

This IgG1 mouse antibody was generated against Bordetella pertussis Adenylate Cyclase toxin and recognizes the distal portion of the catalytic domain (amino acids 373-399).

Highlights:

  • Reacts with the distal portion of the catalytic domain (amino acids 373-399)
  • Suitable for Western Blot and Immunoprecipitation applications

Recombinant versions available at our sister company, Absolute Antibody:

Adenylate Cyclase Toxin (ACT or Cya A) is one of several virulence factors produced by the bacterium Bordetella pertussis. This toxin invades eukaryotic cells and catalyzes the conversion of ATP into cyclic AMP (cAMP).  This leads to inhibition of host cell immune function and macrophage death by apoptosis.  The Adenylate Cyclase protein is composed of a catalytic domain (amino acids 1 to 400), a hydrophobic region (amino acids 500 to 700), a glycine/aspartate-rich repeat unit (amino acids 1000 to 1600), and the C-terminal domain (amino acids 1600-1706).

From the laboratory of Erik L. Hewlett, MD, University of Virginia.

Catalog Number Product Size AVAILABILITY Price Qty
EG8001
Anti-Adenylate Cyclase Toxin [3D1] Antibody
100ug In stock
Regular Price:$352.00
Specifications

Product Type: Antibody
Antigen: Adenylate Cyclase Toxin (ACT, Cya A)
Isotype: IgG1
Clonality: Monoclonal
Clone Name: 3D1
Reactivity: Recognizes the distal portion of the catalytic domain (amino acids 373-399)
Immunogen: Generated against Bordetella pertussis Adenylate Cyclase toxin
Species Immunized: Mouse
Purification Method: protein G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB, IP
Concentration: 1mg/mL
Comments: Recommended for detection of Cya A of B. pertussis origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000) and immunoprecipitation [1–2 µg per 100–500 µg of total protein (1 mL of cell lysate)].
Storage: -20C
Shipped: Cold packs

Data

Proposed epitope map of the 3D1 mAb against AC toxin.

3D1 Figure

The linear sequence of AC toxin is shown along with its four major domains. Solid bars indicate theproposed epitope of the 3D1 MAb.

Adapted from: Lee SJ, Gray MC, Guo L, Sebo P, Hewlet EL. Epitope Mapping of Monoclonal Antibodies against Bordetella pertussis Adenylate Cyclase Toxin. Infect Immun. 67(5): 2090-5 (1999).

Provider
From the laboratory of Erik L. Hewlett, MD, University of Virginia.
References
  1. Lee SJ, Gray MC, Guo L, Sebo P, Hewlet EL. Epitope Mapping of Monoclonal Antibodies against Bordetella pertussis Adenylate Cyclase Toxin. Infect Immun. 67(5): 2090-5 (1999).
  2. Herrou J, Willett JW, Crosson S. Structured and Dynamic Disordered Domains Regulate the Activity of a Multifunctional Anti-σ Factor. MBio. 2015 Jul 28;6(4):e00910. View Article
  3. Luebke JL, Eaton DS, Sachleben JR, Crosson S. Allosteric control of a bacterial stress response system by an anti-σ factor. Mol Microbiol. 2018 Jan;107(2):164-179. View Article
  4. Polak M, Zasada AA, Mosiej E, Krysztopa-Grzybowska K, Witkowski L, Rzeczkowska M, Piekarska K, Lutyńska A. Pertactin-deficient Bordetella pertussis isolates in Poland-a country with whole-cell pertussis primary vaccination. Microbes Infect. 2018 Dec 21. pii: S1286-4579(18)30193-X. View Article

If you publish research with this product, please let us know so we can cite your paper.

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