This IgG1 mouse antibody was generated against Bordetella pertussis Adenylate Cyclase toxin and recognizes the distal portion of the catalytic domain (amino acids 373-399).
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Adenylate Cyclase Toxin (ACT or Cya A) is one of several virulence factors produced by the bacterium Bordetella pertussis. This toxin invades eukaryotic cells and catalyzes the conversion of ATP into cyclic AMP (cAMP). This leads to inhibition of host cell immune function and macrophage death by apoptosis. The Adenylate Cyclase protein is composed of a catalytic domain (amino acids 1 to 400), a hydrophobic region (amino acids 500 to 700), a glycine/aspartate-rich repeat unit (amino acids 1000 to 1600), and the C-terminal domain (amino acids 1600-1706).
From the laboratory of Erik L. Hewlett, MD, University of Virginia.
|Antigen:||Adenylate Cyclase Toxin (ACT, Cya A)|
|Reactivity:||Recognizes the distal portion of the catalytic domain (amino acids 373-399)|
|Immunogen:||Generated against Bordetella pertussis Adenylate Cyclase toxin|
|Purification Method:||protein G|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB, IP|
|Comments:||Recommended for detection of Cya A of B. pertussis origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000) and immunoprecipitation [12 µg per 100500 µg of total protein (1 mL of cell lysate)].|
Proposed epitope map of the 3D1 mAb against AC toxin.
The linear sequence of AC toxin is shown along with its four major domains. Solid bars indicate theproposed epitope of the 3D1 MAb.
Adapted from: Lee SJ, Gray MC, Guo L, Sebo P, Hewlet EL. Epitope Mapping of Monoclonal Antibodies against Bordetella pertussis Adenylate Cyclase Toxin. Infect Immun. 67(5): 2090-5 (1999).
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