This plasmid expresses the full length E. coli Histidyl-tRNA synthetase with a N-terminal 6x polyhistidine-tag.
Histidyl-tRNA synthetases attach amino acids to the 3' ends of their cognate transfer RNAs in a two-step reaction catalyzed by ATP. The accuracy of this reaction is crucial for the overall fidelity of protein synthesis.
From the laboratory of Christopher S. Francklyn, PhD, University of Vermont.
Part of The Investigator's Annexe program.
|Gene/insert name:||E. coliHistidine tRNA Synthetase, 6x His tagged, IPTG inducible|
|Fusion Tag(s):||N-terminal 6x His tag|
|Grow in E. coli at 37 C:||Yes|
|Cloning Site 5':||PstI|
|Cloning Site 3':||HindIII|
|Insert Size:||1272 bp|
|Vector Backbone and Size:||pQE30, 3462 bp|
|High or low copy:||High|
|Shipped:||Ambient temperature (Liquid plasmid DNA in water for domestic orders, spotted on filter paper for international orders)|
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