IRF3 Expressing HEK293 Cell Line (HEK293-IRF3)
Human embryonic kidney (HEK293) cells that stably express Interferon Regulatory Factor 3 (IRF-3) tagged by a Venus fluorescent protein.
Interferon regulatory factors (IRF) are proteins that regulate transcription of interferons (see regulation of gene expression). IRF3 is a key transcription factor to activate the expression of interferons. IRF3 contains several functional domains including a nuclear export signal, a DNA-binding domain, a C-terminal IRF association domain, and several regulatory phosphorylation sites. IRF3 plays an important role in the innate immunity against viral infection. IRF3 has been shown to interact with IRF7. HEK293-IRF3 was established with a stale expression of IRF3 and used in studies of the interferon induction pathway.
From the laboratory of Yanjin Zhang, PhD, University of Maryland, College Park.
Human embryonic kidney (HEK293) cells that stably express Interferon Regulatory Factor 3 (IRF-3) tagged by a Venus fluorescent protein.
Interferon regulatory factors (IRF) are proteins that regulate transcription of interferons (see regulation of gene expression). IRF3 is a key transcription factor to activate the expression of interferons. IRF3 contains several functional domains including a nuclear export signal, a DNA-binding domain, a C-terminal IRF association domain, and several regulatory phosphorylation sites. IRF3 plays an important role in the innate immunity against viral infection. IRF3 has been shown to interact with IRF7. HEK293-IRF3 was established with a stale expression of IRF3 and used in studies of the interferon induction pathway.
From the laboratory of Yanjin Zhang, PhD, University of Maryland, College Park.
| Product Type: | Cell Line |
| Name: | HEK293-IRF3, HEK293-VenusC1-IRF-3 |
| Cell Type: | Human embryonic kidney cells |
| Accession ID: | Q14653 |
| Morphology: | Monolayer cells, Epithelial |
| Organism: | Human |
| Source: | Embryonic kidney |
| Biosafety Level: | BSL 2 |
| Subculturing: | Adherent, 1:3 dilution |
| Growth Conditions: | DMEM with 10% FBS, 1x Penicillin-Streptomycin-Glutamine, 1x sodium pyruvate, and plasmocin of 1 ug/ml |
| Cryopreservation: | Complete growth medium supplemented with 10% DMSO |
| Comments: | HEK293 cells stably expressing VenusC1-IRF-3 (HEK293-IRF-3) were established by transfection of the cells with VenusC1-IRF-3 and selection under the antibiotic G418. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
- Nan, Y., Yu, Y., Ma, Z., Khattar, S.K., Fredericksen, B., Zhang, Y.J., 2014. Hepatitis E virus inhibits type I interferon induction by ORF1 products. J. Virol. 88, 11924-11932.
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