pGSHU Plasmid
Plasmid pGSHU contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter.
From the laboratory of Francesca Storici, PhD*, National Institute of Environmental Health Sciences/NIH.
*Now affiliated with Georgia Institute of Technology.
Plasmid pGSHU contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter.
From the laboratory of Francesca Storici, PhD*, National Institute of Environmental Health Sciences/NIH.
*Now affiliated with Georgia Institute of Technology.
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Specifications
| Product Type: | Plasmid |
| Gene/insert name: | KlURA3, hyg, I-SceI |
| Antibiotic Resistance: | Ampicillin |
| Grow in E. coli at 37 C: | Yes |
| Vector Backbone and Size: | pFA6aKlURA3, 7184 bp |
| Storage: | -20C |
| Shipped: | Room temperature |
Provider
From the laboratory of Francesca Storici, PhD*, National Institute of Environmental Health Sciences/NIH.
*Now affiliated with Georgia Institute of Technology.
Comments
References
- Katz, S., and Storici, F. (2014). Genetic modification stimulated by the induction of a site-specific break distant from the locus of correction in haploid and diploid yeast. In: “Methods in Molecular Biology”, Edited by F. Storici. Humana Press, New York, NY; 1114:308-324.
- Stuckey, S., and Storici, F. (2013) Gene Knockouts, in vivo Site-Directed Mutagenesis and Other Modifications Using the Delitto Perfetto System in Saccharomyces cerevisiae. In Jon Lorsch, editors: Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate, Vol 533, MIE, UK: Academic Press, 2013, pp. 103-132.
- Stuckey, S., Mukherjee, K., and Storici, F. (2011). In vivo site-specific mutagenesis and gene collage using the delitto perfetto system in yeast Saccharomyces cerevisiae. In: “Methods in Molecular Biology”, Edited by: H. Tsubouchi. Humana Press Inc., New York, NY; 745:173-191.
- Storici F, Durham CL, Gordenin DA, Resnick MA. Chromosomal site-specific double-strand breaks are efficiently targeted for repair by oligonucleotides in yeast. Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14994-9. Epub 2003 Nov 20.
- Storici F, Resnick MA. Delitto perfetto targeted mutagenesis in yeast with oligonucleotides. Genet Eng (N Y). 2003;25:189-207. Review.
- Storici F, Lewis LK, Resnick MA. In vivo site-directed mutagenesis using oligonucleotides. Nat Biotechnol. 2001 Aug;19(8):773-6.
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