Leukotriene B4 (LTB4) is produced from leukocytes in response to inflammatory mediators and is able to induce the adhesion and activation of leukocytes on the endothelium, allowing them to bind to and cross into the tissue. In neutrophils, LTB4 is a potent chemoattractant, and is able to induce the formation of reactive oxygen species and the release of lysosome enzymes.
Sample Requirements: Serum, Plasma (EDTA or Heparin) or Culture Supernatants
Minimum Sample Volume: 100uL
From the laboratory of Clifford J. Rosen, PhD, Maine Medical Center Research Institute.
Part of The Investigator's Annexe program.
Note: Animal serum used in the preparation of cell culture media may contain high levels of LTB4. For best results, do not use animal serum for growth of cell cultures when assaying for LTB4 production. If animal serum is used as a supplement in the media, the appropriate media control should be prepared and tested in the immunoassay to determine the baseline concentration of LTB4.
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