Pre-formed fibrils (PFFs) of wild type (WT) alpha-synuclein.
Alpha-synuclein (α-synuclein) is an intrinsically disordered protein of unknown function that is linked to both familial and sporadic cases of Parkinson's disease, where it forms the major constituent of the pathological Lewy body aggregates. These fibrils can be used for disease modelling since similar PFFs have been shown to seed endogenous α-synuclein inclusions in healthy cells.
From the laboratory of Scott D. Ryan, PhD, University of Guelph.
|Name:||pre-formed fibrils (PFFs) of WT alpha-synuclein|
|Alternative Name(s):||alpha-syn-WT PFFs|
|Source:||Human, expressed in E. coli BL21 (DE3) CP+|
|Amino Acid Sequence:||MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA|
|Purity:||>95 %, purified by ion-exchange chromatography followed by reverse phase chromatography|
|Buffer:||Dulbeccos Phosphate Buffered Saline, Sigma D8537|
|Comments:||A sedimentation assay was completed to confirm PFF formation|
Preparing PFFs (Pre-Formed Fibrils) for Treatment of Primary Neurons
SAFETY: Wear gloves, disposable sleeves, and a second pair of gloves when handling PFFs. Dispose of pipette tips and microfuge tubes in a container of 1% SDS. Wear protective headphones while sonicating fibrils, and decontaminate probe and spills with 1% SDS.
In cell culture, these sonicated PFFs are typically used at 1% vol/vol (or 10uL/mL of media).
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