This monoclonal antibody reacts with VSV-G protein from the Indiana serotype.
Vesicular stomatitis virus (VSV) is a well studied, enveloped, negative-strand RNA virus. The VSV genome encodes for 5 proteins: N, P, M, G, and L. The G protein (glycoprotein) is located at the virion surface and is responsible for virus attachment and penetration. Additionally, many lentivrial vectors are pseudotyped with VSV-G from the Indiana serotype.
From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.
|Clone Name:||8G5FL11 (I1)|
|Specificity:||VSV-Ind glycoprotein (G) protein|
|Purity:||Protein G purified|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||Western blot (1:1000), flow cytometry, immunofluorescence|
|Storage:||-20C (avoid repeated freeze / thaw cycles)|
BHK cells were infected with VSV and labeled with Anti-VSV-G (8G5F11) and JacksonLabs Dylight 549 goat anti-mouse secondary antibody. Cells were imaged at 20X using QImaging Camera (half of 14-bit range with < 1000 ms exposure). Cells were stained and imaged live. (contrast adjusted 8-bit image).
Image courtesy of KeraFAST Customer Jay Warrick, Ph.D., University of Wisconsin Madison, submitted November 16, 2011.
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