Recombinant nanobody against Glutathione S-Transfrase (GST) produced in alpaca, expressed in E. coli.
Glutathione S-transferase (GST) tags are often used to increase expression, solubility and to purify recombinant proteins. GST can be fused to either the N-terminus or C-terminus of a protein. This anti-GST single domain antibody (nanobody) is ~13 kDa in size and is more resistant to heat and pH than a conventional antibody. It exhibits the same high affinity as a conventional antibody and can be used for pull-down assays, ELISAs, or other applications with GST-fusion proteins. It is not recommended to be used in Western blots.
From the laboratory of Louis B. Hersh, PhD, University of Kentucky.
|Antigen:||Gluathionine S-transferase (Schistosoma japonicum)|
|Molecular Weight:||26 kDa|
|Clonality:||Single domain antibody (nanobody®)|
|Purification Method:||Affinity chromatography then molecular sieve chromatography|
|Method Used to Determine Concentration:||OD280|
|Comments:||Recombinant His-tagged single domain antibody|
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