The 143B rho-0#25 cell line is a derivative of the 143B human osteosarcoma cells, which have lost their mitochondrial DNA (mtDNA) as a result of transient transfection with plasmid pMA4008 encoding HSV UL12.5M185 protein, which damages mtDNA by an unknown mechanism.
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
|Product Type:||Cell Line|
|Source:||143B cell line|
|Biosafety Level:||BSL 1|
|Subculturing:||Split 1:4 every 3 days|
|Growth Conditions:||DMEM/10% FBS/ 1 mM pyruvate, 50 µg/ml uridine|
|Cryopreservation:||Media + 10% DMSO|
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