143B rho-0#41 Cell Line
The 143B rho-0#41 cell line is a derivative of the 143B human osteosarcoma cells, which have lost their mitochondrial DNA (mtDNA) as a result of transient transfection with plasmid pMA4008 encoding HSV UL12.5M185 protein, which damages mtDNA by an unknown mechanism.
Highlights:
- Useful as recipients in production of cytoplasmic hybrids (cybrids)
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
The 143B rho-0#41 cell line is a derivative of the 143B human osteosarcoma cells, which have lost their mitochondrial DNA (mtDNA) as a result of transient transfection with plasmid pMA4008 encoding HSV UL12.5M185 protein, which damages mtDNA by an unknown mechanism.
Highlights:
- Useful as recipients in production of cytoplasmic hybrids (cybrids)
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
Specifications
| Product Type: | Cell Line |
| Cell Type: | Osteosarcoma |
| Source: | 143B cell line |
| Organism: | Human |
| Morphology: | Epithelial |
| Biosafety Level: | BSL 1 |
| Subculturing: | Split 1:4 every 3 days |
| Growth Conditions: | DMEM/10% FBS/ 1 mM pyruvate, 50 µg/ml uridine |
| Cryopreservation: | Media + 10% DMSO |
| Comments: | Slow- Growing |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Documentation
Provider
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.
References
- Spadafora D, Kozhukhar N, Chouljenko VN, Kousoulas KG, Alexeyev MF. Methods for Efficient Elimination of Mitochondrial DNA from Cultured Cells. PLoS One. 2016 May 2;11(5):e0154684.
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