Tobacco Etch Virus protease (TEVp)-Streptavidin fusion protein immobilized on biotin-coated superparamagnetic nanoparticles for optimal recovery and reuse in protein preparation applications.
Proteases with highly specific activities have applications in the purification and downstream processing of overexpressed proteins, including the cleavage of affinity tags and solubility promoting partners such as GST and MBP. However, proteases can be challenging to express and purify, and commercially sourced proteases such as TEV and 3C can be prohibitively costly.
From the laboratory of Robert M. Hughes, PhD, East Carolina University.
|Product Type:||Small Molecule|
|Name:||TEV Protease-Labelled Magnetic Nanoparticles|
|Functionalization:||Biotin coated NPs are loaded with TEVp-Streptavidin fusion protein|
|Stability:||TEV beads gradually lose activity in cold storage. Activity can be readily restored with the addition of reductant to the proteolysis reaction activity upon reactivation with reductant after storage for two weeks at +4C|
|Mean Diameter:||0.5 microns|
|Comments:||End-users who obtain this product will need a magnet for bead separation|
|Storage:||+4C (do not freeze)|
Suggested reaction conditions: Utilize 50-100 μL bead suspension per 250 μL-1 mL reaction; conduct reactions at 30-34C for 6 h to overnight on rotator. Collect beads on magnet for 5 min prior to recovery of supernatant. Beads are active for > 1 month if stored at 4C. Beads can be reused for multiple protease reactions.
Tip: Add DTT or β-ME to a final concentration of 10 mM for optimal substrate proteolysis.
If you publish research with this product, please let us know so we can cite your paper.