13C6-UV Cleavable Biotin-Azide

This isotopically labeled “heavy” tag can be used in parallel to the unlabeled “light” analog (See: UV Cleavable Biotin-Azide) to screen protein adduction by identifying hits as those with a mass difference of 6 Da.

Highlights:

  • Biotin conjugate can be subsequently visualized by streptavidin Western techniques and/or enriched using streptavidin purification methods
  • Captured target can be released under mild photolysis conditions (365 nm) and results in a significant decrease of background signal due to non-specifically bound proteins

From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.

Catalog Number Product Size AVAILABILITY Price Qty
EVU151
13C6-UV Cleavable Biotin-Azide
3mg In stock
Regular Price:$750.00
Specifications

Product Type: Small Molecule
Name: 13C6-UV-Biotin-azide
Chemical Formula: C3213C6H51N7O9S
Molecular Weight: 787.88
Format: Pale yellow powder
Purity: >95%
Solubility: DMSO, MeOH
Spectral Information: 1H, 13C NMR
Comments: Protect from light and hydrolysis conditions
Storage: Upon receipt store at -20C
Shipped: Ambient temperature

Data
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Provider
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
References
  1. Quantitative Chemoproteomics for Site-Specific Analysis of Protein Alkylation by 4-Hydroxy-2-Nonenal in Cells. Yang, J.; Tallman, K. A.; Porter, N.A.; Liebler, D. C. Analytical Chemistry 2015, 87(5), 2535.
  2. A Chemoproteomic Platform to Assess Bioactivation Potential of Drugs. Sun, R.; Shi, F.; Liu, K.; Fu, L.; Tian, C.; Yang, Yong; Tallman, K. A.; Porter, N. A.; Yang, J. Chemical Research in Toxicology 2017, 30(10), 1797.
  3. Fu L, Liu K, He J, Tian C, Yu X, Yang J. Direct Proteomic Mapping of Cysteine Persulfidation. Antioxid Redox Signal. 2020 Nov 20;33(15):1061-1076. View article

If you publish research with this product, please let us know so we can cite your paper.

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