This mouse IgG2a monoclonal antibody [rJ2], recognizes double stranded RNA (dsRNA) of greater than 40 bp in length which is generated during the replication of positive sense genome viruses.
rJ2 antibody can be used to detect dsRNA intermediates of viruses as diverse as Zika virus, Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine fever virus, Brome mosaic virus and many more in cultured cells and also in fixed paraffin-embedded histological samples.
For more information on how this antibody reacts with Zika virus see a blog post from ASM: Zika Virus Inside Cells
From the laboratory of Abraham L. Brass, MD, PhD, University of Massachusetts Medical School.
|Antigen:||double stranded RNA produced by positive sense genome viruses|
|Molecular Weight:||dsRNA > 40 bp in length|
|Isotype:||IgG2a, kappa light chain|
|Clone Name:||Recombinant J2 (rJ2)|
|Reactivity:||Positive sense genome viruses|
|Epitope:||dsRNA from positive sense genome virus replication|
|Buffer:||Culture medium, DMEM and 5% FBS|
|Tested Applications:||IF (1:60), IHC, immuno-EM, Affinity purification|
Zika Virus Infecting Cells
Anti-dsRNA [rJ2] Antibody used at 1:60 to detect Zika virus (green) infection of cultured human cells. Zika virus levels are lower (top right panel), as UMMS researchers decreased the levels of the EMC1 protein in the human cells using siRNA. NT= non-targeting negative control siRNA. Cell nuclei are shown in blue below
Adapted from: Savidis G, et al. Cell Reports June 21 2016
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