Anti-Pectic Polysaccharide [MH4.2A4] Antibody (supernatant)
![Anti-Pectic Polysaccharide [MH4.2A4] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1067_2001202001263310660.jpg "Anti-Pectic Polysaccharide [MH4.2A4] Antibody (supernatant)")
This mouse IgM monoclonal (MH4.2A4) was generated against tobacco leaf protoplasts and recognizes intact protoplasts/glycoproteins/plasma membrane antigens containing arabinogalactans.
Highlights:
- Reacts with tobacco intact protoplasts/glycoproteins/plasma membrane antigens. Also reacts with many dicot rhamnogalacturonan I preparations.
- Suitable for ELISA applications
Pectin is made up of a complex set of polysaccharides that are found in most primary cell walls and are particularly abundant in the non-woody parts of terrestrial plants. Pectin is a major component of the middle lamella, where it helps to bind cells together, but is also found in primary cell walls.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
This mouse IgM monoclonal (MH4.2A4) was generated against tobacco leaf protoplasts and recognizes intact protoplasts/glycoproteins/plasma membrane antigens containing arabinogalactans.
Highlights:
- Reacts with tobacco intact protoplasts/glycoproteins/plasma membrane antigens. Also reacts with many dicot rhamnogalacturonan I preparations.
- Suitable for ELISA applications
Pectin is made up of a complex set of polysaccharides that are found in most primary cell walls and are particularly abundant in the non-woody parts of terrestrial plants. Pectin is a major component of the middle lamella, where it helps to bind cells together, but is also found in primary cell walls.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
| Product Type: | Antibody |
| Antigen: | Sycamore Pectic Polysaccharides |
| Isotype: | IgM |
| Clonality: | Monoclonal |
| Clone Name: | 2A4.G1.C4 |
| Reactivity: | Reacts with tobacco intact protoplasts/glycoproteins/plasma membrane antigens. Also reacts with many dicot rhamnogalacturonan I preparations. |
| Immunogen: | Protoplast |
| Species Immunized: | Mouse |
| Buffer: | Cell culture supernatant, Use undiluted |
| Tested Applications: | ELISA |
| Storage: | <1 month at 4C, >1 month at -80C |
| Shipped: | Cold Packs |
Notes
MH4.2A4 binds to linseed mucilage and weakly to some other arabinogalactan-containing glycans among the 54 polysaccharides tested. MH4.2A4 also binds to arabinogalactan glycoproteins from diverse plants.
These monoclonal antibodies were developed under the sponsorship of the US National Science Foundation, through award number DBI-0421683. Their use in biomass characterization, study of biomass deconstruction and quantitation was developed under the sponsorship of the US Department of Energy through awards DE-PS02-06ER64304 and DE-AC05-00OR22725 (BioEenergy Science Center).
- Hahn MG, Lerner DR, Fitter MS, Norman PM, Lamb CJ (1987) Characterization of monoclonal antibodies to protoplast membranes of Nicotiana tabacum identified by an enzyme-linked immunosorbent assay. Planta 171:453-465.
- Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.
If you publish research with this product, please let us know so we can cite your paper.
