Diazo Biotin-Azide
An alkyne-reactive, sodium dithionite (Na2S2O4) cleavable biotinylation reagent.
Highlights:
- Cleavable – a diazo spacer arm allows protein targets to be released under mild conditions
- Chemoselective – azide and alkyne groups do not react or interfere with other functional groups found in biological samples, but conjugate to one another with high efficiency
- Solubility – reagent easily dissolves in water-miscible solvents for subsequent dilution into aqueous reaction mixtures
- Extended PEG3 spacer – reduces aggregation, minimizes steric hindrance for affinity-binding to avidin or streptavidin
Diazo Biotin-Azide allows the capture of alkyne-tagged biomolecules via a copper-catalyzed click reaction. This reaction possesses extreme selectivity, such that the complementary reagents can form covalent bonds within richly functionalized biological systems, and, in some cases, living organisms. Biotinylated protein targets are selectively captured through conventional affinity purification protocols that exploit the biotin - streptavidin interaction. Captured protein targets can be release of under mild conditions with 50 mM sodium dithionite solution, and identified by mass spectrometry, ELISA, dot blot, or Western blot applications.
An alkyne-reactive, sodium dithionite (Na2S2O4) cleavable biotinylation reagent.
Highlights:
- Cleavable – a diazo spacer arm allows protein targets to be released under mild conditions
- Chemoselective – azide and alkyne groups do not react or interfere with other functional groups found in biological samples, but conjugate to one another with high efficiency
- Solubility – reagent easily dissolves in water-miscible solvents for subsequent dilution into aqueous reaction mixtures
- Extended PEG3 spacer – reduces aggregation, minimizes steric hindrance for affinity-binding to avidin or streptavidin
Diazo Biotin-Azide allows the capture of alkyne-tagged biomolecules via a copper-catalyzed click reaction. This reaction possesses extreme selectivity, such that the complementary reagents can form covalent bonds within richly functionalized biological systems, and, in some cases, living organisms. Biotinylated protein targets are selectively captured through conventional affinity purification protocols that exploit the biotin - streptavidin interaction. Captured protein targets can be release of under mild conditions with 50 mM sodium dithionite solution, and identified by mass spectrometry, ELISA, dot blot, or Western blot applications.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Small Molecule |
| Name: | Diazo Biotin-Azide |
| Chemical Formula: | C33H45N9O7S |
| Molecular Weight: | 711.83 |
| Variant MPN: | 1041 |
| Format: | Orange solid to grey solid |
| Solubility: | DMSO, DMF |
| Storage: | -20C |
| Shipped: | Ambient |
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