|Name:||Anti-exocyst complex sec15 subunit (15S2G6 ) monoclonal antibody|
|Isotype:||IgG subtype not characterized|
|Specificity:||This antibody recognizes exocyst complex sec15 subunit. Demonstrated to react with rat and mouse tissues.|
|Immunogen:||Recombinant protein corresponding to full-length rat brain sec15 subunit|
|Tested Applications:||Western blotting, ELISA and Immunofluorescence microscopy.|
|Purity:||Protein G purified|
|Storage:||Store at -20C|
(left) Western blot analysis: 10 ug Rat brain and neuroendocrine PC12 cell lysates were resolved on a 8% SDS-polyacrylamide gel, transferred to nitrocellulose membrane and probed with 0.01ug/ml antibody.
(right) Immunofluorescence microcopy: Methanol-fixed neuroendocrine PC12 cells were stained with 0.1ug/ml antibody. For best visualization, the cells were incubated with the primary antibody at 4C overnight.
- Oztan A, Silvis M, Weisz OA, Bradbury NA, Hsu SC, Goldenring JR, Yeaman C, Apodaca G. (2007) Exocyst requirement for endocytic traffic directed toward the apical and basolateral poles of polarized MDCK cells. Mol Biol Cell. 10:3978-3992.
- Wang, S. and Hsu, S.-C. (2003) Immunological Characterization of Exocyst Complex Subunits in Cell Differentiation. Hybridoma 22: 159-164.
- Brymora A, Valova VA, Larsen MR, Roufogalis BD, Robinson PJ (2001) The brain exocyst complex interacts with RalA in a GTP-dependent manner: identification of a novel mammalian Sec3 gene and a second Sec15 gene. J Biol Chem. 276:29792-29797.
- Vogel GF, Klee KM, Janecke AR, Müller T, Hess MW, Huber LA. Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3. J Cell Biol. 2015 Nov 9;211(3):587-604. doi: 10.1083/jcb.201506112. PubMed PMID: 26553929; PubMed Central PMCID: PMC4639860. View Article
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