|Name:||Anti-exocyst sec5 subunit (5S8A10) monoclonal antibody|
|Isotype:||IgG subtype not characterized|
|Specificity:||This antibody recognizes exocyst complex sec5 subunit. Demonstrated to react with rat and mouse tissues.|
|Immunogen:||Recombinant protein corresponding to full-length rat brain sec5 subunit|
|Tested Applications:||Western blotting, ELISA and Immunofluorescence microscopy.|
|Purity:||Protein G purified|
|Storage:||Store at -20C|
Alternative names - Exoc2, mSec5
(left) Western blot analysis: 10 ug Rat brain lysate was resolved on a 8% SDS-polyacrylamide gel, transferred to nitrocellulose membrane and probed with 0.01ug/ml antibody.
(right) Immunofluorescence microcopy: Methanol-fixed neuroendocrine PC12 cells were stained with 0.1ug/ml antibody. For best visualization, the cells were incubated with the primary antibody at 4 OC overnight.
- Wang L, Li G, Sugita S. (2004) RalA-exocyst interaction mediates GTP-dependent exocytosis. J Biol Chem. 279:19875-19881.
- Fukai S, Matern HT, Jagath JR, Scheller RH, Brunger AT (2003) Structural basis of the interaction between RalA and Sec5, a subunit of the sec6/8 complex. EMBO J. 22:3267-78.
- Sugihara K, Asano S, Tanaka K, Iwamatsu A, Okawa K, Ohta Y (2002) The exocyst complex binds the small GTPase RalA to mediate filopodia formation. Nat Cell Biol. 4:73-8.
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