|Name:||Anti-exocyst exo70 subunit (70X13F3) monoclonal antibody|
|Isotype:||IgG subtype not characterized|
|Specificity:||This antibody recognizes exocyst complex exo70 subunit. Reactive against Mouse and Rat.|
|Immunogen:||Recombinant protein corresponding to full-length rat brain exo70 subunit|
|Tested Applications:||Western blotting, ELISA and Immunofluorescence microscopy.|
|Purity:||Protein G purified|
|Storage:||Store at -20C|
(left) Western blot analysis: 10 ug Rat brain lysate was resolved on a 8% SDS-polyacrylamide gel, transferred to nitrocellulose membrane and probed with 0.01ug/ml antibody.
(right) Immunofluorescence microcopy: Methanol-fixed neuroendocrine PC12 cells were stained with 0.1ug/ml antibody. For best visualization, the cells were incubated with the primary antibody at 4 OC overnight.
- Chernyshova Y, Leshchyns'ka I, Hsu SC, Schachner M, Sytnyk V. (2011) The neural cell adhesion molecule promotes FGFR-dependent phosphorylation and membrane targeting of the exocyst complex to induce exocytosis in growth cones. J. Neurosci 31:3522-3535.
- Liu J, Zuo X, Yue P, Guo W (2007) Phosphatidylinositol 4,5-bisphosphate mediates the targeting of the exocyst to the plasma membrane for exocytosis in mammalian cells. Mol Biol Cell. 18:4483-4492.
- Zuo X, Zhang J, Zhang Y, Hsu SC, Zhou D, Guo W. (2006) Exo70 interacts with the Arp2/3 complex and regulates cell migration. Nat Cell Biol. 2006 8:1383-1388.Ewart MA, Clarke M, Kane S, Chamberlain LH, Gould GW. (2005) Evidence for a role of the exocyst in insulin-stimulated Glut4 trafficking in 3T3-L1 adipocytes. J Biol Chem. 280:3812-3816.
- Vega, I. and Hsu, S.-C. (2001) The Exocyst complex associates with microtubules to mediate vesicle targeting and neurite outgrowth. J. Neurosci. 21: 3839-3848.
- Bernabé-Rubio M, Andrés G, Casares-Arias J, Fernández-Barrera J, Rangel L, Reglero-Real N, Gershlick DC, Fernández JJ, Millán J, Correas I, Miguez DG, Alonso MA. Novel role for the midbody in primary ciliogenesis by polarized epithelial cells. J Cell Biol. 2016 Aug 1;214(3):259-73. doi: 10.1083/jcb.201601020. PubMed PMID: 27458130; PubMed Central PMCID: PMC4970324.View Article
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