Anti-p53 [Pab240] Antibody
This mouse IgG1k monoclonal antibody recognizes mammalian and chicken p53 and was generated against a gel purified p53-b-galactosidase fusion protein containing a sequence corresponding to amino acids 14-389 of p53 (derived from the pSV53C p53 cDNA clone).
p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. p53 is crucial in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressor that is involved in preventing cancer. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. Activated p53 binds DNA and activates expression of several genes leading to unregulated cellular proliferation.
From a laboratory at Cancer Research Technology.
Part of The Investigator's Annexe program.
This mouse IgG1k monoclonal antibody recognizes mammalian and chicken p53 and was generated against a gel purified p53-b-galactosidase fusion protein containing a sequence corresponding to amino acids 14-389 of p53 (derived from the pSV53C p53 cDNA clone).
p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. p53 is crucial in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressor that is involved in preventing cancer. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. Activated p53 binds DNA and activates expression of several genes leading to unregulated cellular proliferation.
From a laboratory at Cancer Research Technology.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Name: | Anti-p53 (Pab 240) monoclonal antibody |
| Accession ID: | P04637 |
| Host: | Balb/c |
| Isotype: | IgG1k |
| Clonality: | Monoclonal |
| Clone Name: | Pab240 |
| Specificity: | Mammals and chicken |
| Immunogen: | A gel purified p53-b-galactosidase fusion protein containing p53 sequence from amino acids 14-389 (derived from the pSV53C p53 cDNA clone) |
| Format: | Liquid |
| Purification Method: | Protein G purified |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | FACS, Immunoprecipitation, Paraffin embedded, WB |
| Concentration: | 1mg/mL |
| Amount: | 100uL |
| Storage: | Store at 4C |
| Shipped: | Cold packss |
Detection of p53 using PAb 240: 50% colon cancer sections are positive, 30% of breast cancer sections and 70% of lung cancer sections. Normal and premalignant tissue are negative.
- Iggo R, Gatter K, Bartek J, Lane D, Harris AL. Increased expression of mutant forms of p53 oncogene in primary lung cancer. 1990. Lancet. 335:675-9. PMID: 1969059
- Gannon JV, Greaves R, Iggo R, Lane DP. Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form. 1990. EMBO J. 9:1595-602. PMID: 1691710
- Bartek J, Bartkova J, Vojtesek B, Staskova Z, Lukas J, Rejthar A, Kovarik J, Midgley CA, Gannon JV, Lane DP. Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies. 1991. Oncogene. 6:1699-703. PMID: 1923535
- Said JW, Barrera R, Shintaku IP, Nakamura H, Koeffler HP. Immunohistochemical analysis of p53 expression in malignant lymphomas. 1992. Am. J Pathol. 141:1343-8. PMID: 1466398
- Walker RA, Dearing SJ, Lane DP, Varley JM. Expression of p53 protein in infiltrating and in-situ breast carcinomas. 1991. J Pathol. 165:203-11. PMID: 1684809
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