Recombinant TEV Protease

Recombinant TEV protease, used in the cleavage of affinity tags, recognizes the consensus sequence ENLYFQ\S, where "\" is the cleavage site.

TEV protease (EC 3.4.22.44, Tobacco Etch Virus nuclear-inclusion-a endopeptidase) is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV). It is a member of the PA clan of chymotrypsin-like proteases. Due to its high sequence specificity it is frequently used for the controlled cleavage of fusion proteins in vitro and in vivo.

From the laboratory of David L. Blum, PhD and Michelle M. Lewis, PhD, University of Georgia.

Catalog Number Product Size AVAILABILITY Price Qty
EGA311
Recombinant TEV Protease, 1.8mg
1.8mg (18mg/mL) In stock
Price: $100.00
Specifications
Product Type: Protein
Name: Tobacco Etch Virus nuclear-inclusion-a endopeptidase (TEV) Protease
EC Number: EC 3.4.22.44
CAS number: 139946-51-3
Accession ID: M15239.1; P04517
Fusion Tag(s): Histidine tag
Buffer: 25 mM NaPO4 pH 7.6, 200 mM NaCl, 10% glycerol and 2mM DTT
Source: Purified from overexpression in E. coli
Purity: >80%
Concentration: 0.7uM; 18.8mg/mL; 100uL
Activity: >95% cleavage at 2uM for 2hrs at room temperature
Comments: Recognition sequence: Glu-Asn-Leu-Tyr-Phe-Glu-\-Ser
Storage: -80C, avoid multiple freeze-thaw cycles
Shipped: Dry Ice
Provider
From the laboratory of David L. Blum, PhD and Michelle M. Lewis, PhD, University of Georgia.
References
  1. Rawlings ND, Barrett AJ, Bateman A (January 2012). "MEROPS: the database of proteolytic enzymes, their substrates and inhibitors". Nucleic Acids Res. 40 (Database issue): D343–50. doi:10.1093/nar/gkr987. PMC 3245014 Freely accessible. PMID 22086950.

If you publish research with this product, please let us know so we can cite your paper.

 
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