Recombinant TEV Protease
Recombinant TEV protease, used in the cleavage of affinity tags, recognizes the consensus sequence ENLYFQ\S, where "\" is the cleavage site.
TEV protease (EC 3.4.22.44, Tobacco Etch Virus nuclear-inclusion-a endopeptidase) is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV). It is a member of the PA clan of chymotrypsin-like proteases. Due to its high sequence specificity it is frequently used for the controlled cleavage of fusion proteins in vitro and in vivo.
From the laboratory of David L. Blum, PhD and Michelle M. Lewis, PhD, University of Georgia.
Recombinant TEV protease, used in the cleavage of affinity tags, recognizes the consensus sequence ENLYFQ\S, where "\" is the cleavage site.
TEV protease (EC 3.4.22.44, Tobacco Etch Virus nuclear-inclusion-a endopeptidase) is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV). It is a member of the PA clan of chymotrypsin-like proteases. Due to its high sequence specificity it is frequently used for the controlled cleavage of fusion proteins in vitro and in vivo.
From the laboratory of David L. Blum, PhD and Michelle M. Lewis, PhD, University of Georgia.
Specifications
| Product Type: | Protein |
| Name: | Tobacco Etch Virus nuclear-inclusion-a endopeptidase (TEV) Protease |
| EC Number: | EC 3.4.22.44 |
| CAS number: | 139946-51-3 |
| Accession ID: | M15239.1; P04517 |
| Source: | Purified from overexpression in E. coli |
| Fusion Tag(s): | Histidine tag |
| Purity: | >80% |
| Buffer: | 25 mM NaPO4 pH 7.6, 200 mM NaCl, 10% glycerol and 2mM DTT |
| Concentration: | 5mg/mL |
| Activity: | >95% cleavage at 2uM for 2hrs at room temperature |
| Comments: | Recognition sequence: Glu-Asn-Leu-Tyr-Phe-Glu-\-Ser |
| Storage: | -80C, avoid multiple freeze-thaw cycles |
| Shipped: | Dry Ice |
Provider
From the laboratory of David L. Blum, PhD and Michelle M. Lewis, PhD, University of Georgia.
References
- Rawlings ND, Barrett AJ, Bateman A (January 2012). "MEROPS: the database of proteolytic enzymes, their substrates and inhibitors". Nucleic Acids Res. 40 (Database issue): D343–50. doi:10.1093/nar/gkr987. PMC 3245014 Freely accessible. PMID 22086950.
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