Anti-CD6 [34-3] Antibody

This rat IgG2b monoclonal antibody was raised against full length CDNA, whole CD6-transfected NB2 cells and is specific for mouse T-cell differentiation antigen CD6.

Highlights:

  • Reacts with mouse CD6
  • Binds to the extracellular domain of CD6
  • Suitable for Flow Cytometry applications

T-cell differentiation antigen CD6 is a cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses. It has been shown to interact with ALCAM/CD166. Cd6 is found on the outer membrane of T-lymphocytes as well as some other immune cells. CD6 contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. It is important protein for continuation of T cell activation.

From the laboratories of Jane R. Parnes, MD and Susan Prohaska, PhD, Stanford University.

Catalog Number Product Size AVAILABILITY Price Qty
ESU001
Anti-CD6 [34-3] Antibody, 100ug
100ug 3-5 weeks
Price: $299.00
Specifications
Product Type: Antibody
Antigen: mouse CD6
Accession ID: Q61003
Molecular Weight: 130 kDa
Isotype: IgG2b
Clonality: Monoclonal
Clone Name: 34-3
Reactivity: Mouse
Immunogen: Protein
Species Immunized: Rat
Epitope: Extracellular domain
Purification Method: Protein A/G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: Flow Cytometry (1 µg detects CD6 in one million mouse splenocytes)
Storage: -20C
Shipped: Cold Packs
Data

Tissue distribution of mouse CD6 detected with anti-mCD6 mAb #34-3.

Panel A shows anti-CD6 co-staining CD3+ (top row) and CD5+ (bottom row) lymphocytes from peripheral blood, lymph node, spleen and thymus. Panel B shows CD6 expression on CD4+ (top row) or CD8+ (bottom row) T cells from peripheral blood, lymph node, spleen and thymus.

Provider
From the laboratories of Jane R. Parnes, MD and Susan Prohaska, PhD, Stanford University.
Comments
Spleen cells were then fused by standard methods with the mouse myeloma line, Ag8.653, and plated out in 96-well plates in complete DMEM supplemented with HEPES, 20% fetal calf serum, 20% thymocyte conditioned medium, and HAT (hypoxanthine, aminopterine, thymidine) (Sigma, St. Louis, MO). Supernatants from wells that were positive for growth under HAT selection were screened by flow cytometry for anti-CD6 reactivity against the CD6-transfected NB2 rat T cell line. Clone #34 was found to be IgG2b. mAb #34 was subcloned once again and the subclone designated #34-3 was used for subsequent studies.
References

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