pFA6A-GFP-ura4MX6 Plasmid
The pFA6A-GFP-ura4MX6 plasmid is used to perform genomic GFP-tagging to express a C-terminally GFP-fused protein in the fission yeast Schizosaccharomyces pombe.
Highlights:
- A shuttle plasmid that can be propagated in E. coli and S. pombe
- Useful to facilitate imaging analyses of proteins
Schizosaccharomyces pombe, also called "fission yeast", is a species of yeast used in traditional brewing and as a model organism in molecular and cell biology. Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.
From the laboratory of Eishi Noguchi, PhD, Drexel University.
The pFA6A-GFP-ura4MX6 plasmid is used to perform genomic GFP-tagging to express a C-terminally GFP-fused protein in the fission yeast Schizosaccharomyces pombe.
Highlights:
- A shuttle plasmid that can be propagated in E. coli and S. pombe
- Useful to facilitate imaging analyses of proteins
Schizosaccharomyces pombe, also called "fission yeast", is a species of yeast used in traditional brewing and as a model organism in molecular and cell biology. Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.
From the laboratory of Eishi Noguchi, PhD, Drexel University.
| Product Type: | Plasmid |
| Gene/insert name: | ura4MX6 |
| Antibiotic Resistance: | Ampicillin |
| Fusion Tag(s): | GFP |
| Grow in E. coli at 37 C: | Yes |
| Selectable markers: | Ampicillin for E. coli; ura4 for S. pombe |
| Cloning Site 5': | PmeI |
| Cloning Site 3': | BglII |
| Insert Size: | 1769 bp |
| Vector Backbone and Size: | 3447 bp |
| High or low copy: | High copy in E. coli |
| Comments: | Kan in pFA6a-GFP-kanMX (Bähler et al, Yeast. 1998 Jul, 14(10):943-51.) was replaced with ura4. |
| Storage: | -20C |
| Shipped: | Room Temperature |
- Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E. New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe. Biotechniques. 2013 Nov;55(5):257-63.
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