Zika Virus Plasmids

pJW231 and pJW232 plasmids offer an infections clone system that produce virus with in vitro and in vivo characteristics similar to those of the primary isolate, providing a critical tool to study ZIKV infection and disease.

Highlights:

  • Two-plasmid infectious clone system was created, from which infectious virus was rescued that replicates in human and mosquito cells with growth kinetics representative of wild-type ZIKV
  • pJW231 (Plasmid 1) - Encodes ZIKV 5’ end (1-3498)
  • pJW232 (Plasmid 2) - Encodes ZIKV 3’ end (3,109-10,809) and HDV Ribozyme
  • Infectious clone-derived virus initiated infection and transmission rates in Aedes aegypti mosquitoes comparable to those of the primary isolate and displayed similar pathogenesis in AG129 mice

Zika virus (ZIKV; family Flaviviridae, genus Flavivirus) is a rapidly expanding global pathogen that has been associated with severe clinical manifestations, including devastating neurological disease in infants. This ZIKV reverse genetics system based on a 2015 isolate from Puerto Rico (strain PRVABC59). This infectious clone produces virus with in vitro and in vivo characteristics similar to those of the primary isolate, providing a critical tool to study ZIKV infection and disease.

From the laboratories of Brian J. Geiss, PhD and Gregory D. Ebel, ScD, Colorado State University.

Catalog Number Product Size AVAILABILITY Price Qty
ECS001
pJW231 Zika Virus Plasmid 1
Spotted on filter paper In stock
Price: $66.00
ECS002
pJW232 Zika Virus Plasmid 2
Spotted on filter paper In stock
Price: $66.00
Specifications
Product Type: Plasmid
Gene/insert name: pJW231 (aka p1-ZIKV): T7 Promoter/Zika virus (strain PRVABC59) 5' end (1-3498)
pJW232 (aka p2-ZIKV): Zika virus (strain PRVABC59) 3' end (3,109-10,809) and HDV Ribozyme
Fusion Tag(s): None
Antibiotic Resistance: Ampicillin
Grow in E. coli at 37 C: DH5-alpha E. Coli or similar. Can grow at 37C.
Selectable markers: None
Cloning Site 5': pJW231: ApaLI
pJW232: SapI
Cloning Site 3': pJW231: PmlI
pJW232: PvuI
Insert Size: pJW231: 3834 bp
pJW232: 9458 bp
Vector Backbone and Size: pACYC177
High or low copy: Low
Comments: Using chloramphenicol amplification can increase plasmid yield.
Storage: -20C
Shipped: Room Temperature
Provider
From the laboratories of Brian J. Geiss, PhD and Gregory D. Ebel, ScD, Colorado State University.
References
  1. Weger-Lucarelli J, Duggal NK, Bullard-Feibelman K, Veselinovic M, Romo H, Nguyen C, Rückert C, Brault AC, Bowen RA, Stenglein M, Geiss BJ, Ebel GD. Development and Characterization of Recombinant Virus Generated from a New World Zika Virus Infectious Clone. J Virol. 2016 Dec 16;91(1). pii: e01765-16. Print 2017 Jan 1. Erratum in: J Virol. 2017 Mar 29;91(8):. PubMed PMID: 27795432

If you publish research with this product, please let us know so we can cite your paper.

 
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