Anti-Fli1b Antibody

This rabbit polyclonal antibody was generated against recombinant protein and is specific for zebrafish Fli1b protein (Fli1b).

Highlights:

  • Reacts with zebrafish Fli1b
  • Binds to the N-terminal half of Fli1b
  • Suitable for Western Blot and Whole Mount Immunostaining applications

Fli1b protein is a transcription factor that is essential for vascular differentiation and morphogenesis in vertebrates.

From the laboratory of Nathan D. Lawson, PhD, University of Massachusetts Medical School.

Catalog Number Product Size AVAILABILITY Price Qty
ES1005
Anti-Fli1b Antibody, 100uL
100uL In stock
Price: $299.00
Specifications
Product Type: Antibody
Antigen: Fli1b
Accession ID: NP_001008780
Molecular Weight: 51 kDa
Clonality: Polyclonal
Reactivity: Zebrafish
Immunogen: Recombinant protein
Species Immunized: Rabbit
Epitope: N-terminal half of Fli1b
Buffer: Polyclonal serum with 0.02% sodium azide
Tested Applications: WB (1:5000), Whole mount immunostaining (1:500)
Comments: Recommend aliquotting into small working volumes after initial thaw and store these at -80°C
Storage: -80C
Shipped: Dry Ice
Data

Immunohistochemistry

Upper panel: Whole mount immunohistochemistry, using 17-24 hours post fertilization wild type zebrafish embryos (2%PFA fixed). Primary stain: anti-Fl1b rabbit polyclonal antibody (1:500 dilution). Secondary stain: Invitrogen Goat anti-rabbit Alexa fluor 488 (Cat#:A111034); [1:1,000 Dilution].

Lower panel: Whole mount immunohistochemistry of 48 hours post fertilization zebrafish embryos, visualizing head (left) and trunk (right) regions. Primary stain: anti-Fli1b rabbit polyclonal antibody (1:500 dilution). Secondary stain: Invitrogen donkey anti-rabbit Alexa fluor 568 (Cat#:A10042); [1:1,000 Dilution].

Provider
From the laboratory of Nathan D. Lawson, PhD, University of Massachusetts Medical School.

References
  1. Moore JC, Sheppard-Tindell S, Shestopalov IA, Yamazoe S, Chen JK, Lawson ND. Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. Dev Biol. 2013 Dec 1;384(1):128-40.
  2. Villefranc JA, Nicoli S, Bentley K, Jeltsch M, Zarkada G, Moore JC, Gerhardt H, Alitalo K, Lawson ND. A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development. Development. 2013 Apr;140(7):1497-506.

If you publish research with this product, please let us know so we can cite your paper.

 
Loading...
Loading...