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Specifications
| Product Type: | Antibody |
| Antigen: | Fli1b |
| Accession ID: | NP_001008780 |
| Molecular Weight: | 51 kDa |
| Clonality: | Polyclonal |
| Reactivity: | Zebrafish |
| Immunogen: | Recombinant protein |
| Species Immunized: | Rabbit |
| Epitope: | N-terminal half of Fli1b |
| Buffer: | Polyclonal serum with 0.02% sodium azide |
| Tested Applications: | WB (1:5000), Whole mount immunostaining (1:500) |
| Comments: | Recommend aliquotting into small working volumes after initial thaw and store these at -80°C |
| Storage: | -80C |
| Shipped: | Dry Ice |
Data
Immunohistochemistry
Upper panel: Whole mount immunohistochemistry, using 17-24 hours post fertilization wild type zebrafish embryos (2%PFA fixed). Primary stain: anti-Fl1b rabbit polyclonal antibody (1:500 dilution). Secondary stain: Invitrogen Goat anti-rabbit Alexa fluor 488 (Cat#:A111034); [1:1,000 Dilution].
Lower panel: Whole mount immunohistochemistry of 48 hours post fertilization zebrafish embryos, visualizing head (left) and trunk (right) regions. Primary stain: anti-Fli1b rabbit polyclonal antibody (1:500 dilution). Secondary stain: Invitrogen donkey anti-rabbit Alexa fluor 568 (Cat#:A10042); [1:1,000 Dilution].
Provider
From the laboratory of Nathan D. Lawson, PhD, University of Massachusetts Medical School.
References
- Moore JC, Sheppard-Tindell S, Shestopalov IA, Yamazoe S, Chen JK, Lawson ND. Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. Dev Biol. 2013 Dec 1;384(1):128-40.
- Villefranc JA, Nicoli S, Bentley K, Jeltsch M, Zarkada G, Moore JC, Gerhardt H, Alitalo K, Lawson ND. A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development. Development. 2013 Apr;140(7):1497-506.
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