Anti-Etv2 Antibody

This rabbit polyclonal antibody was generated against recombinant protein and is specific for zebrafish ETS Variant 2 (Etv2).

Highlights:

  • Reacts with zebrafish Etv2
  • Binds to the N-terminal half of Etv2
  • Suitable for Western Blot and Whole Mount Immunostaining applications

Etv2 also known as Ets-related protein 71 is an endothelial-specific ETS transcription factor that is essential for vascular differentiation and morphogenesis in vertebrates.

From the laboratory of Nathan D. Lawson, PhD, University of Massachusetts Medical School.

Catalog Number Product Size AVAILABILITY Price Qty
ES1004
Anti-Etv2 Antibody, 100uL
100uL In stock
Price: $299.00
Specifications
Product Type: Antibody
Antigen: Etv2
Accession ID: NP_001032452
Molecular Weight: 41 kDa
Clonality: Polyclonal
Reactivity: Zebrafish
Immunogen: Recombinant protein
Species Immunized: Rabbit
Epitope: N-terminal half of Etv2
Buffer: Polyclonal serum with 0.02% sodium azide
Tested Applications: WB (1:5000), Whole mount immunostaining (1:500)
Comments: Recommend aliquotting into small working volumes after initial thaw and store these at -80°C
Storage: -80C
Shipped: Dry Ice
Data

Immunohistochemistry

Whole mount immunohistochemistry, using 12 or 17 hours post fertilization wild type zebrafish embryos (2%PFA fixed). Primary stain: anti-Etv2 rabbit polyclonal antibody (1:500 dilution). Secondary stain: Invitrogen Goat anti-rabbit Alexa fluor 488 (Cat#:A111034); [1:1,000 Dilution].

Western Blot

Western blot of HEK293T cell lysates cotransfected with a 6xc-Myc tagged version of Etv2 and Enhanced green fluorescent protein (EGFP). Lysates where run on a TRIS-Glycine gel and transfered to PDVF membrane. The blot was cut, blocked with 5% dried milk and probed individually with a 1:5,000 dilution of anti-Etv2 polyclonal antibody, 1:10,000 dilution of Sigma anti-c-Myc monoclonal antibody(clone: 9E10), or 1:5,000 dilution of Invitrogen anti-GFP rabbit polyclonal (Cat#:A11122). A 1:20,000 dilution of species specific HRP conjugated secondary was used for visualization.

Adapted from: Moore JC et al., Dev Biol. 2013 Dec 1;384(1):128-40.

Provider
From the laboratory of Nathan D. Lawson, PhD, University of Massachusetts Medical School.

References
  1. Moore JC, Sheppard-Tindell S, Shestopalov IA, Yamazoe S, Chen JK, Lawson ND. Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. Dev Biol. 2013 Dec 1;384(1):128-40.

If you publish research with this product, please let us know so we can cite your paper.

 
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