CD20-GFP Expressing BJAB Cell Line
BJAB cells expressing green fluorescence protein (GFP)-B-lymphocyte antigen CD20.
Highlights:
- Cells express GFP and CD20
CD20, or B-lymphocyte antigen CD20, is a cell-surface protein expressed in most immune cells arising from the B-cell lineage and plays a role in cellular activation and proliferation. Importantly, CD20 is a recognized biomarker for a number of human disorders, including several autoimmune diseases and B-cell malignancies. BJAB cells come from a patient with Burkitt lymphoma, which is a form of non-Hodgkin's lymphoma in which cancer starts in immune cells called B-cells. GFP was fused to the amino terminus of CD20 by inserting human CD20 cDNA into the cloning site of the pEGFP expression vector using XhoI/SacII. Stable transfectants of BJAB cells expressing green fluorescence protein (GFP)-CD20 were generated by transfection and selection using G418 and cell sorting.
From the laboratory of Julie P. Deans, PhD, University of Calgary.
BJAB cells expressing green fluorescence protein (GFP)-B-lymphocyte antigen CD20.
Highlights:
- Cells express GFP and CD20
CD20, or B-lymphocyte antigen CD20, is a cell-surface protein expressed in most immune cells arising from the B-cell lineage and plays a role in cellular activation and proliferation. Importantly, CD20 is a recognized biomarker for a number of human disorders, including several autoimmune diseases and B-cell malignancies. BJAB cells come from a patient with Burkitt lymphoma, which is a form of non-Hodgkin's lymphoma in which cancer starts in immune cells called B-cells. GFP was fused to the amino terminus of CD20 by inserting human CD20 cDNA into the cloning site of the pEGFP expression vector using XhoI/SacII. Stable transfectants of BJAB cells expressing green fluorescence protein (GFP)-CD20 were generated by transfection and selection using G418 and cell sorting.
From the laboratory of Julie P. Deans, PhD, University of Calgary.
| Product Type: | Cell Line |
| Cell Type: | Lymphoid |
| Accession ID: | P11836 |
| Source: | Patient with Burkitt's lymphoma |
| Organism: | Human |
| Morphology: | Typical lymphoid |
| Biosafety Level: | BSL 2 |
| Growth Conditions: | RPMI 10% FBS, with geneticin 0.4 mg/mL if needed |
| Cryopreservation: | 10% DMSO and 90% FBS |
| Comments: | GFP was fused to the amino terminus of CD20 by inserting human CD20 cDNA into the cloning site of the pEGFP expression vector using XhoI/SacII. Stable transfectants of BJAB cells expressing green fluorescence protein (GFP)-CD20 were generated by transfection and selection using G418 and cell sorting. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
- Li H, Ayer LM, Polyak MJ, Mutch CM, Petrie RJ, Gauthier L, Shariat N, Hendzel MJ, Shaw AR, Patel KD, Deans JP. The CD20 calcium channel is localized to microvilli and constitutively associated with membrane rafts: antibody binding increases the affinity of the association through an epitope-dependent cross-linking-independent mechanism. J Biol Chem. 2004 May 7;279(19):19893-901.
If you publish research with this product, please let us know so we can cite your paper.
