P2Y Receptor Expressing Human Astrocytoma Cell Lines

1321N1 human astrocytoma cell lines expressing various P2Y purinergic G protein-coupled receptors.

Highlights:

  • Available expressing one of the following: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, or P2Y14
  • Distinguished from most other cultured cell lines in that native 1321N1 cells have no response to extracellular nucleotides
  • Most other cell lines express one or more P2Y receptors, making them problematic for screening or assessing receptor function

Astrocytomas are a type of cancer of the brain. They originate in a particular kind of glial cells, star-shaped brain cells in the cerebrum called astrocytes. This type of tumor does not usually spread outside the brain and spinal cord and it does not usually affect other organs. P2Y receptors are a family of purinergic G protein coupled receptors that are stimulated by nucleotides.

From the laboratory of Robert A. Nicholas, PhD, University of North Carolina at Chapel Hill.

Catalog Number Product Size AVAILABILITY Price Qty
ENC018
P2Y1 Expressing Human Astrocytoma Cell Line (1321N1-HA-P2Y1), 1 vial
1 vial 3-5 weeks
Price: $599.00
ENC019
P2Y2 Expressing Human Astrocytoma Cell Line (1321N1-HA-P2Y2), 1 vial
1 vial 3-5 weeks
Price: $599.00
ENC020
P2Y4 Expressing Human Astrocytoma Cell Line (1321N1-HA-P2Y4), 1 vial
1 vial 3-5 weeks
Price: $599.00
ENC021
P2Y6 Expressing Human Astrocytoma Cell Line (1321N1-HA-P2Y6), 1 vial
1 vial 3-5 weeks
Price: $599.00
ENC022
P2Y11 Expressing Human Astrocytoma Cell Line (1321N1-HA-P2Y11), 1 vial
1 vial 3-5 weeks
Price: $599.00
ENC023
P2Y12 Expressing Human Astrocytoma Cell Line (1321N1-HA-P2Y12), 1 vial
1 vial 3-5 weeks
Price: $599.00
ENC024
P2Y13 Expressing Human Astrocytoma Cell Line (1321N1-HA-P2Y13), 1 vial
1 vial 3-5 weeks
Price: $599.00
ENC025
P2Y14 Expressing Human Astrocytoma Cell Line (1321N1-HA-P2Y14), 1 vial
1 vial 3-5 weeks
Price: $599.00
Specifications
Product Type: Cell Line
Name: See different names (receptors) above
Cell Type: Glioblastoma
Organism: Human
Morphology: Adherent cells
Biosafety Level: BSL 2
Subculturing: Split sub-confluent cultures (90%) 1:6 to 1:10 using trypsin/EDTA
Growth Conditions: Grown in 5% CO2 in high-glucose Dulbecco's modified Eagle's medium supplemented with 5% fetal bovine serum, 100 U/ml penicillin, and 0.1 mg/ml streptomycin.
Cryopreservation: 10% DMSO and 90% FBS
Source: Glial Cell
Comments: Slower growing cells, adherent
Storage: LN2
Shipped: Dry Ice
Provider
From the laboratory of Robert A. Nicholas, PhD, University of North Carolina at Chapel Hill.

Comments

Receptor Information:

  • The P2Y1 receptor couples to Gq and is important in ADP-mediated platelet aggregation and activated by ADP
  • The P2Y2 receptor couples to Gq and has been reported to also couple to Go and G12 and equipotently activated by UTP and ATP
  • The P2Y4 receptor is coupled to Gq and activated by UTP; the human receptor is antagonized by ATP
  • The P2Y6 receptor is activated by UDP and is coupled to Gq
  • The P2Y11 receptor is activated by ATP and couples to Gq and Gs, although the coupling to Gq occurs at lower concentration of agonist
  • The P2Y12 receptor is important in platelet aggregation and is the target of clopidogrel and other platelet inhibitors; The receptor coupels to Gi
  • The P2Y13 receptor is activated by ADP and couples to Gi proteins
  • The P2Y14 receptor is activated by UDP-glucose and UDP and couples to Gi family proteins
References
  1. Filtz, T, Q Li, JL Boyer, RA Nicholas, and TK Harden (1994) Cloning, expression, and pharmacological characterization of a P2Y-purinergic receptor from turkey brain. Mol Pharm, 46:8-14.
  2. Schachter, J, Li, Q, Boyer, J, Nicholas, RA, and Harden, TK (1996) Second messenger cascade specificity and pharmacological selectivity of the human P2Y1-purinoceptor. Brit J Pharmacol, 118:167-173.
  3. Nicholas, RA, Watt, WC, Lazarowski, ER, Li, Q, and Harden, TK (1996) The uridine nucleotide selectivity of three phospholipase C-activating P2 receptors: Identification of a UDP-selective, a UTP-selective, and an adenine and uridine triphosphate-specific receptor. Mol Pharmacol, 50:224-229.
  4. Kennedy, C, Qi, A-D, Herold, CL, Harden, TK and Nicholas, RA (2000) ATP, an agonist at the rat P2Y4 receptor, is an anatagonist at the human P2Y4 receptor. Mol Pharmacol 57:926-931.
  5. Qi, A-D, Kennedy, C, Harden, TK and Nicholas, RA (2001) Differential coupling of the human P2Y11 receptor to phospholipase C and adenylyl cyclase. Brit J Pharmacol 132: 318-326.
  6. Chhatriwala M, Ravi RG, Patel RI, Boyer JL, Jacobson KA, Harden TK. Induction of novel agonist selectivity for the ADP-activated P2Y1 receptor versus the ADP-activated P2Y12 and P2Y13 receptors by conformational constraint of an ADP analog. J Pharmacol Exp Ther. 2004 Dec;311(3):1038-43.
  7. Lazarowski ER, Harden TK. UDP-Sugars as Extracellular Signaling Molecules: Cellular and Physiologic Consequences of P2Y14 Receptor Activation. Mol Pharmacol. 2015 Jul;88(1):51-60.

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