Rat Cochlear Microglial Cell Line (Mocha)
Mocha cells are a rat cochlear microglial cell line. They were developed from an immunomagnetically enriched population of CD11b+ cells from P3 rat cochlea and immortalized with the 12S E1A gene of adenovirus in a replication-incompetent retroviral vector.
Highlights
- Express markers consistent with microglia (CD68, CD11b, Iba1, F4/80, and TMEM119)
- Respond to LPS stimulation by upregulation of genes (Cox2, ICAM-1, Il6r, Ccl2, Il13Ra, and Il15Ra) as well as release of cytokines (IL-1beta, IL-12, IL-13 and RANTES)
- Phagocytose fluorescent beads at 37C, a hallmark of microglial function
- Have undergone verification by IDEXX-RADIL to be of rat origin without contamination by other mammalian cell types. A baseline genetic profile of these cells is available here: Seigel, et al. Mol. Cell. Neurosci. 85 (2017) 202-210.
Microglia are a unique lineage of mononuclear phagocytic cells that play an essential role in development, immunological defense and in- flammatory processes in the central nervous system. Microglia account for 10-15% of all cells found within the brain. As the resident macrophage cells, they act as the first and main form of active immune defence in the central nervous system (CNS).
From the laboratory of Gail M. Seigel, PhD, University at Buffalo.
Mocha cells are a rat cochlear microglial cell line. They were developed from an immunomagnetically enriched population of CD11b+ cells from P3 rat cochlea and immortalized with the 12S E1A gene of adenovirus in a replication-incompetent retroviral vector.
Highlights
- Express markers consistent with microglia (CD68, CD11b, Iba1, F4/80, and TMEM119)
- Respond to LPS stimulation by upregulation of genes (Cox2, ICAM-1, Il6r, Ccl2, Il13Ra, and Il15Ra) as well as release of cytokines (IL-1beta, IL-12, IL-13 and RANTES)
- Phagocytose fluorescent beads at 37C, a hallmark of microglial function
- Have undergone verification by IDEXX-RADIL to be of rat origin without contamination by other mammalian cell types. A baseline genetic profile of these cells is available here: Seigel, et al. Mol. Cell. Neurosci. 85 (2017) 202-210.
Microglia are a unique lineage of mononuclear phagocytic cells that play an essential role in development, immunological defense and in- flammatory processes in the central nervous system. Microglia account for 10-15% of all cells found within the brain. As the resident macrophage cells, they act as the first and main form of active immune defence in the central nervous system (CNS).
From the laboratory of Gail M. Seigel, PhD, University at Buffalo.
| Product Type: | Cell Line |
| Name: | Mocha (short for microglia of the cochlea) |
| Cell Type: | Microglia of the cochlea |
| Accession ID: | CVCL_QX29 |
| Morphology: | Mixed microglial (both amoeboid and partially ramified) |
| Source: | Cochlea |
| Organism: | P3 Sprague-Dawley rat |
| Biosafety Level: | BSL-2 |
| Subculturing: | Cells are adherent with doubling time of about 24 hours, Passage 3X per week with 0.125% trypsin-EDTA |
| Growth Conditions: | DMEM with 10% calf serum at 37C in 5% CO2 |
| Cryopreservation: | DMEM/10% calf serum with 10% DMSO |
| Comments: | Mocha cells are resistant to neomycin/G418 |
| Storage: | Liquid Nitrogen |
| Shipped: | Dry Ice |
View Dr. Seigel's presentation describing the Mocha cell line here.
- Seigel GM, Manohar S, Yingying, B, Ding D and Salvi R. An immortalized microglial cell line (Mocha) derived from rat cochlea. Mol. Cell. Neurosci. 85 (2017) 202-210. View Article
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