Human Bronchial Epithelial Cell Lines

Immortalized human bronchial epithelial cell lines for cystic fibrosis research.

Highlights

  • Non-CF and CF cell lines produced similar levels of IL-8 at baseline and increased IL-8 secretion in response to IL-1beta, TNFalpha and the Toll-like receptor 2 agonist, Pam3Cys
  • Cell lines are similar to primary hBE cells in their fastidious requirements for growth factors, substratum, adequate seeding density and good husbandry practices

Bmi-1 is a proto-oncogene that maintains stem cells by suppressing cyclin dependent kinase inhibitors, and its expression creates cell lines that recapitulate normal cell structure and function. Using lentiviral vectors, we introduced Bmi-1 and the catalytic subunit of telomerase (hTERT) into three different non-Cystic Fibrosis (CF) and 3 ΔF508 homozygous CF primary bronchial cell preparations. Bmi-1/hTERT expression extended cell lifespan while maintaining a diploid karyotype. When seeded at high density on porous supports, passage 15 cells exhibited a pseudostratified morphology with few ciliated cells but abundant mucus secretory cells. Ussing Chamber studies demonstrated electrophysiologic properties mostly comparable to early passage primary cells.

From the laboratory of Scott H. Randell, PhD, University of North Carolina at Chapel Hill.

Catalog Number Product Size AVAILABILITY Price Qty
ENC011
Human Bronchial Epithelial Cell Line (UNCN1T)
1 vial 3-5 weeks
Price: $699.00
ENC012
Human Bronchial Epithelial Cell Line (UNCN2T)
1 vial 3-5 weeks
Price: $699.00
ENC013
Human Bronchial Epithelial Cell Line (UNCN3T)
1 vial 3-5 weeks
Price: $699.00
ENC014
Cystic Fibrosis Human Bronchial Epithelial Cell Line (UNCCF1T)
1 vial 3-5 weeks
Price: $699.00
ENC015
Cystic Fibrosis Human Bronchial Epithelial Cell Line (UNCCF2T)
1 vial 3-5 weeks
Price: $699.00
ENC016
Cystic Fibrosis Human Bronchial Epithelial Cell Line (UNCCF3T)
1 vial 3-5 weeks
Price: $699.00
Specifications
Product Type: Cell Line
Cell Type: Bronchial Epithelial Cell Lines
Organism: Human
Morphology: Packed cuboidal morphology grown in BEGM
Biosafety Level: BSL-2
Subculturing: Expand in flask/dish until 70-90% confluent using BEGM. Trypsinize and expand as desired. Seed onto collagen coated porous inserts for Air-Liquid-Interface cultures
Growth Conditions: Grow at 37C/5%CO2. Basal Epithelial Growth Medium (BEGM) for expansion and Air-Liquid-Interface Medium (ALI) for well-differentiated cultures. See: PDF  Human CF/Bronchial Epithelial Cell Line Protocol
Cryopreservation: 2% 1.5M Hepes, 10% FBS, 78% F12(1X), 10% DMSO
Source: Lung Bronchus from normal and cystic fibrosis
Storage: Liquid Nitrogen
Shipped: Dry Ice
Data

Demographics of tissue donors

Cell Line Name:
Age, yr:
Sex:
Race:
Smoking:
Cause of Death or Diagnosis:
UNCN1T
47
M
Cau
Ns
Head injury
UNCN2T
39
F
Cau
18 pack years
Cerebral hemorrhage
UNCN3T
16
F
Cau
Ns
Head injury
UNCCF1T
33
M
Cau
Ns
CF, ΔF508/ΔF508
UNCCF2T
18
F
Cau
Ns
CF, ΔF508/ΔF508
UNCCF3T
54
F
Cau
Ns
CF, ΔF508/ΔF508

M, male; F, female; Cau, Caucasian; NS, nonsmoker. Non-cystic fibrosis (CF) lungs were obtained from organ donors whose lungs were unsuitable for transplant due to acute injury or lack of a matching recipient. CF lung tissues were obtained at transplantation; the CF patient genotype was obtained from the medical record and was confirmed by a second round of PCR and sequencing of resulting CF cell.

Adapted from: Fulcher ML, et al. Am J Physiol Lung Cell Mol Physiol. 2009 Jan;296(1):L82-91.

Provider
From the laboratory of Scott H. Randell, PhD, University of North Carolina at Chapel Hill.

References
  1. Fulcher ML, Gabriel SE, Olsen JC, Tatreau JR, Gentzsch M, Livanos E, Saavedra MT, Salmon P, Randell SH. Am J Physiol Lung Cell Mol Physiol. 2009 Jan;296(1):L82-91. doi: 10.1152/ajplung.90314.2008. Epub 2008 Oct 31.

If you publish research with this product, please let us know so we can cite your paper.

 
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