Marek's Disease Kidney Cell Line (MDCC-UD35)

MDCC (Marek's disease cell culture) UD35 cells were established from a kidney-localized lymphoma using the RB-1B strain of Marek's disease virus (MDV).

Marek's disease is a highly contagious viral neoplastic disease in chickens. Marek's disease is caused by an alphaherpesvirus known as 'Marek's disease virus' (MDV) or Gallid herpesvirus 2 (GaHV-2). The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes.

From the laboratory of Mark S. Parcells, PhD, University of Delaware.

Catalog Number Product Size AVAILABILITY Price Qty
EDW009
Marek's Disease Kidney Cell Line (MDCC-UD35)
1 Vial 2-3 weeks
Price: $550.00
Specifications
Product Type: Cell Line
Cell Type: Chicken T-cells (CD4hi, TCR2hi, CD3hi, CD28lo, CD29hi, CD30hi, CD44hi, CD45lo, CD164lo, MHC-Ihi, MHC-IIlo)
Organism: Chicken, Gallus gallus, (SPF single-comb white leghorn)
Morphology: non-adherent, pleomorphic, lymphoblastoid
Biosafety Level: BSL-1
Subculturing: 1:5 to 1:10 every 2 - 3 days
Growth Conditions: Established using: Iscove?s modified Dulbecco minimal essential medium (IMDMEM) supplemented with 20% FBS,10% chicken serum, 10% tryptose-phosphate broth, 4 mM L-glutamine, 2 µM 2-mercaptoethanol, 1X nonessential amino acids, 1X insulin / transferrin / selenium (ITS), 1X Pen/Strept/Neo, 1X fungizone. Adapted to: DMEM, high glucose, 10% FBS, 4 mM L-glutamine, 1 X PSN, 1X fungizone
Cryopreservation: 90% FBS/10% DMSO
Source: Kidney lymphoma
Comments: Grow at 41C, 5% CO2. When actively growing, double every 12 -24 hrs. Grow at 5 x 105 to 2 x 107 cells/ml.
Storage: Liquid nitrogen
Shipped: Dry Ice
Provider
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
References
  1. Kumar, P, H Dong, D Lenihan, S Gaddamanugu, U Katneni, S Shaikh, P Tavlarides-Hontz, SM Reddy, W Peters, and MS Parcells. 2012. Selection of a recombinant Marek's disease virus in vivo through expression of the Marek's EcoRI-Q (Meq)-encoded oncoprotein: characterization of an rMd5-based mutant expressing the Meq of strain of RB-1B. Avian Dis. 56:328-340.

If you publish research with this product, please let us know so we can cite your paper.

 
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