Heat-Labile Enterotoxin (HLT) LT-IIa

Type II heat-labile enterotoxin, LT-IIa, from Escherichia coli was produced recombinantly in E. coli and purified to homogeneity using a combination of nickel affinity and gel filtration chromatography.

LT-IIa is a type II heat-labile enterotoxin produced by certain strains of Escherichia coli. The enterotoxin binds to GD1b, GD1a, GM1, and to several other gangliosides on the cell surface. The molecule induces high levels of cAMP in the cell upon intoxication. The molecule is also a very strong mucosal and systemic adjuvant when administered by peritoneal, intranasal, or intradermal routes. Single point substitution mutants of LT-IIa are available from Dr. T.D. Connell, upon request, that have altered ganglioside-binding and toxic activities.

From the laboratory of Terry D. Connell, PhD, University at Buffalo.

Catalog Number Product Size AVAILABILITY Price Qty
EBU028
Heat-Labile Enterotoxin (HLT) LT-IIa, 50ug
50ug In stock
Price: $199.00
Specifications
Product Type: Protein
Name: LT-IIa (A subunit + five B subunits)
Molecular Weight: 82 kDa
Fusion Tag(s): Protein has been His-tagged at the COOH ends of each of the five B subunits.
Format: Powder
Purity: >95%
Solubility: >5mg/mL in water (pH 7.0 to 7.4)
Storage: -20C (long term);+4C when dissolved in aqueous solution (pH 7.0 to 7.4)
Shipped: Room Temperature
Provider
From the laboratory of Terry D. Connell, PhD, University at Buffalo.
References
  1. H.F. Nawar, S. Arce, M.W. Russell, and T.D. Connell. 2005. Mucosal Adjuvant Properties of Mutant LT-IIa and LT-IIb Enterotoxins That Exhibit Altered Ganglioside-Binding Activities. INFECTION AND IMMUNITY 73:1330–1342.

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