Triclosan Resistant (mFabI) Cloning Plasmid

This plasmid is an empty cloning vector containing a novel selectable marker: mFabI, a naturally occurring mutant form of endogenous FabI gene that confers bacteria resistance to an antibacterial compound, triclosan.

  • Triclosan is an appealing option when the products are used in medical applications where residual antibiotics pose an issue
  • mFabI vectors facilitates cloning of challenging sequences by suppressing undesirable sequence recombination events in the host
  • Cost effectiveness of the selection agent brings economic advantage for large scale production of recombinant proteins

Triclosan is a cheap industrially produced biocide widely used in household products. The cost effectiveness of the selection agent brings economic advantage for large scale production of recombinant proteins. In addition, being a non-antibiotic chemical, triclosan is an appealing option when the products are used in medical applications where residual antibiotics pose an issue. In our research, we also found mFabI vectors have a unique property that facilitates cloning of challenging sequences by suppressing undesirable sequence recombination events in the host.

From the laboratory of Terry Magnuson, PhD, University of North Carolina Chapel Hill.

Catalog Number Product Size AVAILABILITY Price Qty
ENC006
Triclosan Resistant (mFabI) Cloning Plasmid, 100ng
100ng In stock
Price: $66.00
Specifications
Product Type: Plasmid
Name: Triclosan Resistant (mFabI) Cloning Plasmid
Organism: Prokaryotic selectable marker
Fusion Tag(s): None
Antibiotic Resistance: 1uM triclosan (non-antibiotic biocide)
Grow in E. coli at 37 C: 32C preferred on plate, 37C in liquid culture
Selectable markers: mFabI
Cloning Site 5': SacI/SacII/NotI/XbaI/SpeI/BamHI
Cloning Site 3': XmaI/SmaI/EcoRI/EcoRV/ClaI/SalI/XhoI/KpnI
Insert Size: No insert present
Vector Backbone and Size: 3253bp
High or low copy: High
Storage: -20C
Shipped: Room Temperature as liquid
Provider
From the laboratory of Terry Magnuson, PhD, University of North Carolina Chapel Hill.
References
  1. Jang CW, Magnuson T. A novel selection marker for efficient DNA cloning and recombineering in E. coli. PLoS One. 2013;8(2):e57075.

If you publish research with this product, please let us know so we can cite your paper.

 
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