Anti-Ricin A Chain [RAC18] Antibody

This mouse IgG2a monoclonal antibody was raised against ricin A chain and is specific for Ricinus communis ricin toxin A chain.

Highlights:

  • Recognizes Ricinus communis ricin toxin A chain
  • Useful for antigen-capture ELISA when used in combination with RBC11 Antibody
  • Suitable for ELISA, Immunofluorescence and Neutralization applications

Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity).

From the laboratory of Seth H. Pincus, MD, Montana State University.

Catalog Number Product Size AVAILABILITY Price Qty
EMT011
Anti-Ricin A Chain [RAC18] Antibody
100ug 2-3 weeks
Price: $306.00
Specifications
Product Type: Antibody
Antigen: Ricin toxin A chain
Accession ID: P02879
Isotype: IgG2a
Clonality: Monoclonal
Clone Name: RAC18
Reactivity: Ricinus communis
Immunogen: Ricin A chain
Species Immunized: Mouse
Epitope: For epitope mapping, see: Maddaloni et al, J. Immunol 2004, 172:6221-28.
Purification Method: Protein G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: Antigen capture ELISA for ricin toxin, immunofluorescence, 1-10µg/ml, Neutralization of ricin toxin in vitro and in vivo, effective concentrations dependent on ricin exposure
Comments: For validation of Ag-capture assay for ricin toxin, see: Simon et al, Toxins, 2015, 7:4967-86
Storage: -20C
Shipped: Cold packs
Provider
From the laboratory of Seth H. Pincus, MD, Montana State Univseristy.
References
  1. Maddaloni M, Cooke C, Wilkinson R, Stout AV, Eng L, Pincus SH. Immunological characteristics associated with the protective efficacy of antibodies to ricin. J Immunol. 2004 May 15;172(10):6221-8.
  2. Simon S, Worbs S, Avondet MA, Tracz DM, Dano J, Schmidt L, Volland H, Dorner BG, Corbett CR. Recommended Immunological Assays to Screen for Ricin-Containing Samples. Toxins (Basel). 2015 Nov 26;7(12):4967-86.

If you publish research with this product, please let us know so we can cite your paper.

 
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