Rat Epineurial Cells

Primary cells isolated from the epineurium layer of the adult rat sciatic nerve.

Highlights:

  • Cells are identified as S100 negative, GFAP negative, p75NGFR negative cells with a variable proportion of Thy-1 positive fibroblasts
  • Highly proliferative and expandable for direct use or cryopreservation
  • Thawing and re-plating of epineurial cells does not alter their viability or other phenotypic characteristics of the cells
  • Cells can be used to study their effects on nerve regeneration
  • They can be used as negative or reference controls in Schwann cell studies performed in vitro and in vivo

Primary cultures of epineurial cells can be used in a variety of applications, as it is becoming increasingly clear that these cells may have a beneficial role in nerve regeneration. Epineurial cells can be used along with Schwann cells to test the cell type specificity of the cellular responses in experiments carried out in vitro and in vivo. The availability of highly viable, cryopreserved stocks of epineurial cells can enable investigators to use the cells themselves or the products derived therefrom (such as DNA/RNA, proteins, exosomes, other) directly in scientific research. Due to their high proliferation rate and expandability, the cells have potential for continued culture, cryopreservation and used as platforms for drug or genetic screens.

From the laboratory of Paula V. Monje, PhD, University of Miami.

Catalog Number Product Size AVAILABILITY Price Qty
EMI012
Rat Epineurial Cells
1M cells/vial 2-3 weeks
Price: $699.00
Specifications
Product Type: Cell Line
Cell Type: Primary epineurial cells from adult rat sciatic nerve tissue
Organism: Rat - 3 months old female
Morphology: Typical fibroblastic morphology, expanded, adherent
Biosafety Level: BSL-1
Subculturing: 2-3 additional passages (recommended). Extensive passaging is possible but the effect on phenotypic stability has not been tested
Growth Conditions: DMEM high glucose containing 10 % decomplemented fetal bovine serum (FBS) and GlutamaxTM. Addition of antibiotics (penicillin, streptomycin, and gentamicin) does not impair viability.
Cryopreservation: RecoveryTM or 90% FBS containing 10% DMSO
Source: Sciatic nerve epineurium
Comments: - Exhibit typical morphological features of cultured fibroblasts and high proliferative capicity. Some heterogeneity in both the shape and size of individual cells may be apparent.
- Cells have a tendency to migrate and spontaneously form aggregates that resemble spheroids when plated at high densities or after reaching confluency. The effect of extended passaging on these populations has not been tested. Other properties of the cells have not yet been investigated.
Storage: Liquid nitrogen
Shipped: Dry Ice
Data

Microscopy Image

Cultures of adult epineurial cells from rat sciatic nerve. Upper panels: representative cultures immunostained with antibodies against Thy-1 (left panel, low magnification image) or Thy-1 and S100 (right panel, high magnification image)to reveal the presence of fibroblasts (Thy-1 positive, green) and absence of contaminating Schwann cells (S100 positive, red). Lower panels: high magnification view of a culture stained with Thy-1 antibodies (green) and EdU (which labels proliferating cells, red). Blue channel: DAPI (nuclear stain). Bottom right panel: phase contrast microscopy of a representative culture 3 days after plating.

Adapted from: Andersen et al. Scientific Reports 6, 31781.

Provider
From the laboratory of Paula V. Monje, PhD, University of Miami.
Comments
Primary adult rat epineurial cells are obtained from the cell populations that result from immediate enzymatic dissociation of the dissected epineurium layer from the adult rat sciatic nerve. Cells (adherent) are cultured in vitro for a time period of 7-12 days to achieve cell expansion prior to subjecting them to cryopreservation. Primary epineurial cells are recognized by their usually expanded morphology and lack of expression of Schwann cell-specific markers. The populations contain a variable proportion of Thy-1 positivecells. Contamination with Schwann cells is negligible and is reduced with passage. Cryopreservation, thawing and re-plating of epineurial cell stocks do not alter the viability or other basic phenotypic characteristics of the cells.
References
  1. Andersen N, Srinivas S, Piñero G, Monje P.V. (2016) A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves. Scientific Reports 6, 31781; doi: 10.1038/srep31781.

If you publish research with this product, please let us know so we can cite your paper.

 
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