Trk SH-SY5Y Neuroblastoma Cell Lines

Immortalized SH-SY5Y cell lines expressing Trk-A or Trk-B. Control Trk-NULL cell line also available.

Highlights

  • SH-SY5Y Neuroblastoma cell lines expressing Trk-A or Trk-B
  • SH-SY5Y Trk-NULL cell line also available

Neuroblastoma (NB) is a tumor of the peripheral nervous system in children, and several receptor tyrosine kinase (RTK) genes have been implicated in malignant transformation or progression of these tumors, including ALK, NTRKs, RET, EGFR and IGFR. The Trk family of neurotrophin receptors (TrkA encoded by NTRK1, TrkB encoded by NTRK2, TrkC encoded by NTRK3) have been shown to play critical roles in regulating both favorable and unfavorable clinical behavior in NBs. TrkA is the receptor for nerve growth factor (NGF), and high TrkA expression is associated with clinically and biologically favorable tumors that have a propensity to undergo spontaneous regression or differentiation. TrkB is the cognate receptor for brain-derived neurotrophic factor (BDNF), and high expression of TrkB plus BDNF is found in the majority of high-risk NBs, especially those with MYCN amplification.

From the laboratory of Garrett M. Brodeur, MD, Children's Hospital of Philadelphia.

Catalog Number Product Size AVAILABILITY Price Qty
ECP004
SH-SY5Y Parental Cell Line
1 vial In stock
Price: $484.00
ECP005
SH-SY5Y Cell Line (pLNCX2 Empty Vector)
1 vial In stock
Price: $484.00
ECP006
SH-SY5Y Cell Line (TrkA, P23A)
1 vial In stock
Price: $484.00
ECP007
SH-SY5Y Cell Line (TrkB, BR6)
1 vial In stock
Price: $484.00
Specifications
Product Type: Cell Line
Name: SH-SY5Y Parental Cell Line
SH-SY5Y Cell Line (pLNCX2 Empty Vector)
SH-SY5Y Cell Line (TrkA, P23A)
SH-SY5Y Cell Line (TrkB, BR6)
Cell Type: Epithelial
Organism: Human
Morphology: Flat, adherent
Biosafety Level: BSL-2
Subculturing: Versene treatment OR low concentrated Trypsin (0.25%)
Growth Conditions: RPMI-1640, 10% FBS, Pen/Strep, 0.3mg/mL G418 (for Trk-A and Trk-B cell lines)
Cryopreservation: 5% DMSO 95% FBS
Source: Neuroblastoma, adrenal
Comments: 18-24 hr doubling time
Storage: Liquid nitrogen
Shipped: Dry Ice
Provider
From the laboratory of Garrett M. Brodeur, MD, Children's Hospital of Philadelphia.
References
  1. Iyer R, Wehrmann L, Golden RL, Naraparaju K, Croucher JL, MacFarland SP, Guan P, Kolla V, Wei G, Cam N, Li G, Hornby Z, Brodeur GM. Entrectinib is a potent inhibitor of Trk-driven neuroblastomas in a xenograft mouse model. Cancer Lett. 2016 Mar 28;372(2):179-86.
  2. Iyer R, Croucher JL, Chorny M, Mangino JL, Alferiev IS, Levy RJ, Kolla V, Brodeur GM. Nanoparticle delivery of an SN38 conjugate is more effective than irinotecan in a mouse model of neuroblastoma. Cancer Lett. 2015 May 1;360(2):205-12.

If you publish research with this product, please let us know so we can cite your paper.

 
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