Rat Primary Schwann Cells

Primary Schwann cells isolated from adult rat sciatic nerve and purified via magnetic activated cell sorting.

Highlights

  • Highly pure (>98%) Schwann cells - Identified on the basis of double immunostaining with the Schwann cell-specific markers, S100 and p75NGFR
  • Exhibit typical elongated (bipolar) phenotype and capacity to express myelin-associated genes upon stimulation with differentiating factors
  • Adhere to substrate and extend processes within 1-2 hours after plating and continue to actively proliferate in the presence of mitogenic factors
  • Expandable through successive rounds of subculture in vitro for at least 3-4 additional passages without losing phenotypic characteristics or biological activity

The culture of primary Schwann cells provides a unique in vitro system for basic research, preclinical drug discovery, neurotoxicity testing, and in vitro modeling of aging, cancer and disease conditions affecting the myelin sheaths. Schwann cell transplantation in experimental animals remains a promising strategy to investigate mechanisms of repair and myelination in the central and peripheral nervous system.

From the laboratory of Paula V. Monje, PhD, University of Miami.

Catalog Number Product Size AVAILABILITY Price Qty
EMI010
Rat Primary Schwann Cells, 1 vial
1 vial 1-2 weeks
Price: $699.00
Specifications
Name: Rat Primary Schwann Cells
Cell Type: Primary adult peripheral nerve Schwann cells
Organism: Rat
Morphology: Elongated, spindle-shaped
Biosafety Level: BSL-1
Growth Conditions: DMEM / 10% FBS, 10 nM neuregulin, 2 µM forskolin
Cryopreservation: 10% DMSO / 90% FBS
Source: Sciatic nerve
Amount: 106 cells/mL
Storage: Liquid nitrogen
Shipped: Dry Ice
Data

Schwann Cell Identification

Purified rat Schwann cells double immunostained with antibodies against S100 (red) and p75NGFR (green). Nuclei were counterstained with (blue).

Provider
From the laboratory of Paula V. Monje, PhD, University of Miami.
Comments

Schwann cells are obtained from the sciatic nerve of adult 3 month old rats using a novel protocol for immediate dissociation and purification by magnetic activated cell sorting (MACS). Schwann cells maintain their identity, viability and growth rates throughout the process of isolation, purification, and expansion and cryopreservation. The cryopreserved product consists of MACS-purified rat Schwann cells identified as S100 positive, p75NGFR positive, GFAP positive cells. The absence of fibroblast contamination is determined on the basis of the lack of expression of Thy-1, a fibroblast-specific marker. These cells actively proliferate in response to defined mitogenic factors and increase the expression of myelination-associated genes in response to cAMP treatment, which are key indicators of biological activity preservation.

Schwann cell cultures established from cryopreserved stocks are viable (>90% at 24 hs post-plating), expandable, homogeneous Schwann cell populations exhibiting typical features of cultured Schwann cells such as ability to align to one another forming typical bundles of cells. Cells can be subcultured in media containing neuregulin and forskolin as mitogenic factors. NOTE: Excesive passaging is not recommended due to possibly phenotypic derivation typically without transformation. Cells can be cryopreserved at essentially any passage.

References
  1. Andersen ND, Srinivas S, Piñero G, Monje PV. A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves. Sci Rep. 2016 Aug 23;6:31781. doi: 10.1038/srep31781. View Article

If you publish research with this product, please let us know so we can cite your paper.

 
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