|Name:||Rat Primary Schwann Cells|
|Cell Type:||Primary adult peripheral nerve Schwann cells|
|Growth Conditions:||DMEM / 10% FBS, 10 nM neuregulin, 2 µM forskolin|
|Cryopreservation:||10% DMSO / 90% FBS|
Schwann cells are obtained from the sciatic nerve of adult 3 month old rats using a novel protocol for immediate dissociation and purification by magnetic activated cell sorting (MACS). Schwann cells maintain their identity, viability and growth rates throughout the process of isolation, purification, and expansion and cryopreservation. The cryopreserved product consists of MACS-purified rat Schwann cells identified as S100 positive, p75NGFR positive, GFAP positive cells. The absence of fibroblast contamination is determined on the basis of the lack of expression of Thy-1, a fibroblast-specific marker. These cells actively proliferate in response to defined mitogenic factors and increase the expression of myelination-associated genes in response to cAMP treatment, which are key indicators of biological activity preservation.
Schwann cell cultures established from cryopreserved stocks are viable (>90% at 24 hs post-plating), expandable, homogeneous Schwann cell populations exhibiting typical features of cultured Schwann cells such as ability to align to one another forming typical bundles of cells. Cells can be subcultured in media containing neuregulin and forskolin as mitogenic factors. NOTE: Excesive passaging is not recommended due to possibly phenotypic derivation typically without transformation. Cells can be cryopreserved at essentially any passage.
- Andersen ND, Srinivas S, Piñero G, Monje PV. A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves. Sci Rep. 2016 Aug 23;6:31781. doi: 10.1038/srep31781. View Article
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