|Subculturing:||1:4 recommended with 0.25% trypsin|
|Growth Conditions:||DMEM high glucose, 15% FBS, 1% NEAA, 4 mM glutamine (penicillin and streptomycin optional)|
|Cryopreservation:||50% FBS, 40% growth media with 10% DMSO|
Cell line 4230 - Contains long expanded GAA repeats on both alleles of the FXN gene: GAA1 ~870 repeats, GAA2 ~1470 repeats. Low level of FXN mRNA expression, 15 22% (depending on the assay used) of average FXN mRNA levels in control cells.
Cell line 4654 - Contains short expanded GAA repeats on both alleles of the FXN gene: GAA1 ~190 repeats, GAA2 ~500 repeats. Moderate/high level of FXN mRNA expression, 35 51% (depending on the assay used) of average FXN mRNA levels in control cells.
- Li Y, Polak U, Clark AD, Bhalla AD, Chen YY, Li J, Farmer J, Seyer L, Lynch D, Butler JS, Napierala M. Establishment and Maintenance of Primary Fibroblast Repositories for Rare Diseases-Friedreich's Ataxia Example. Biopreserv Biobank. 2016 Aug;14(4):324-9. doi: 10.1089/bio.2015.0117.
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