Anti-LINE-1 Retrotransposable Element ORF2 Protein [1G4E11] Antibody

This mouse IgG1k monoclonal antibody was generated against bacterially-purified human endonuclease of open reading frames 2 (ORF2) and is specific or human Long Interspersed Nuclear Element-1 (LINE-1) ORF2.

Highlights:

  • Recognizes human LINE-1 ORF2
  • Suitable for Western Blot applications

LINE-1 retrotransposable element ORF2 protein (ORF2p, LINE1 (also L1 and LINE-1) has a reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition. LINE1 also posesses endonuclease activity, allowing for the introduction of nicks in the chromosomal target DNA. The protein cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to sites of LINE-1 integration in the genome.

From the laboratory of Victoria P. Belancio, PhD, Tulane University.

Catalog Number Product Size AVAILABILITY Price Qty
ETL001
Anti-LINE-1 Retrotransposable Element ORF2 Protein [1G4E11] Antibody, 100ug
100ug In stock
Price: $306.00
Specifications
Antigen: LINE-1 ORF2, L1 ORF2
Accession ID: AH002566, O00370
Molecular Weight: 149 kDa
Clonality: Monoclonal
Reactivity: Human
Immunogen: Bacterially-purified human endonuclease of ORF2
Species Immunized: Mouse
Purification Method: Protein G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB (1:250)
Storage: -20C
Shipped: Cold Packs
Provider
From the laboratory of Victoria P. Belancio, PhD , Tulane University.

References
  1. Sokolowski M, DeFreece CB, Servant G, Kines KJ, deHaro DL, Belancio VP. Development of a monoclonal antibody specific to the endonuclease domain of the human LINE-1 ORF2 protein. Mob DNA. 2014 Dec 10;5(1):29.

If you publish research with this product, please let us know so we can cite your paper.

 
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