Anti-Phytochrome [Oat-24] Antibody

This mouse monoclonal antibody was generated against protein/peptide and recognizes the phytochrome of angiosperms.

Highlights:

  • Recognizes Avena. Pissum phytochrome
  • Possibly applicable to a wide range of angiosperms
  • Useful for the study of the role of phytochromes and mapping their specific antigens in phytochromes
  • Relevent to plant biology and plant biotechnology, especially in the manipulation of light utilization by plants
  • Suitable for Immunoprecipitation, ELISA and Western Blot applications

Phytochrome is a photomorphogenically active pigment that modulates plant growth and development with respect to incident light intensity and wavelength distribution. It exists in two forms: an inactive, red-absorbing form (Pr),4 and an active far-red-absorbing form (Pfr). When either absorbs light, it is photoconverted to the other. Phytochrome is a dimeric, water-soluble, relatively labile chromoprotein with similar, if not identical, monomers of about 124 kDa each. It is also a relatively low abundance protein, even under the best of conditions. Genetic manipulation of phytochrome expression in plants leads to plants requiring less light and able to divert more energy to the production of fruits and seeds. For its physicochemical characterization, it has therefore been difficult to utilize techniques that require large quantitites of highly purified protein. Consequently, indirect methods for elucidating its structure/function relationships are especially important. These could also be applicable to fabaceae and closely related families.

From the laboratories of Lee H. Pratt, PhD and Marie-Michelle Cordonnier Pratt, PhD, University of Georgia.

Catalog Number Product Size AVAILABILITY Price Qty
EGA221
Anti-Phytochrome [Oat-24] Antibody, 5mL (supernatant)
5mL 3-5 weeks
Price: $306.00
Specifications
Product Type: Antibody
Antigen: Phytochrome
Molecular Weight: 124 kDa
Clonality: Monoclonal
Clone Name: Oat-24
Reactivity: Avena. Pissum. Very likely other graminae and fabaceae and beyond.
Immunogen: Protein/ peptide
Species Immunized: Mouse
Epitope: Located at 36 kDa from N-terminus
Buffer: Cell Culture Supernatant
Tested Applications: ELISA, WB, IF, IP, Competitive ELISA
Storage: -80C
Shipped: Dry Ice
Provider
From the laboratories of Lee H. Pratt, PhD and Marie-Michelle Cordonnier Pratt, PhD, University of Georgia.
References
  1. Cordonnier MM, Smith C, Greppin H, Pratt LH. Production and purification of monoclonal antibodies to Pisum and Avena phytochrome. Planta. 1983 Aug;158(4):369-76.
  2. Pratt LH, Cordonnier MM, Lagarias JC. Mapping of antigenic domains on phytochrome from etiolated Avena sativa L. by immunoblot analysis of proteolytically derived peptides. Arch Biochem Biophys. 1988 Dec;267(2):723-35.
  3. M.M. Cordonnier, H. Greppin and Lee H. Pratt (1986) Identification of a Highly Conserved Domain on Phytochrome from Angiosperms to Algae. Plant Physiol. 80, 982-987
  4. CORDONNIER, M.-M., GREPPIN, H., AND PRATT, L. H. (1986), Phytochrome from Green Avena Shoots Characterized with a Monoclonal Antibody to Phytochrome from Etiolated Pisum Shoots, Biochemistry 25,7657-7666.
  5. Shimazaki Y, Cordonnier MM, Pratt LH. Identification with Monoclonal Antibodies of a Second Antigenic Domain on Avena Phytochrome that Changes upon Its Photoconversion. Plant Physiol. 1986 Sep;82(1):109-13.
  6. Cordonnier MM, Greppin H, Pratt LH. Identification of a highly conserved domain on phytochrome from angiosperms to algae. Plant Physiol. 1986 Apr;80(4):982-7.
  7. Shimazaki Y, Cordonnier MM, Pratt LH. Identification with Monoclonal Antibodies of a Second Antigenic Domain on Avena Phytochrome that Changes upon Its Photoconversion. Plant Physiol. 1986 Sep;82(1):109-13.

If you publish research with this product, please let us know so we can cite your paper.

 
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