Anti-Argonaute-2 (AGO2) [4F9] Antibody

This mouse IgG1 monoclonal antibody was generated from a BALB/cJ mouse primed with pristane and immunized with full-length soluble recombinant human Argonaute-2 (Ago2). This antibody is able to immunoprecipitate human Ago2, as well as Ago1, Ago3, and Ago4 (reference 1).

Highlights:

  • Recognizes human Ago2, but also Ago1, Ago3, and Ago4
  • Most suitable for immunoprecipitation and ELISA applications but also used in special application in immunofluorescence
  • Demonstrated use in immunoprecipitation of microRNAs that are associated with Ago2 (reference 1 and 2)

Argonaute (Ago) proteins are the key components that binds siRNA and micrRNA in the RNA induced silencing complex (RISC) complex with molecular masses of approximately 100 kDa and containing PAZ and PIWI domains. Four of these, Ago1 to Ago4, have been demonstrated to associate with miRNAs in humans, however, only Ago2 has been demonstrated to possess the activity of siRNA-guided mRNA cleavage.

From the laboratory of Edward K. Chan, PhD, University of Florida.

Catalog Number Product Size AVAILABILITY Price Qty
EUF001
Anti-Argonaute-2 (AGO2) [4F9] Antibody, 100ug
100ug 2-3 weeks
Price: $204.00
Specifications
Antigen: Human Argonaute-2
Accession ID: AGO2
Molecular Weight: 100 kDa
Isotype: IgG1
Clonality: Monoclonal
Clone Name: 4F9
Reactivity: Human
Immunogen: Pristane-priming and full length soluble recombinant human Ago2
Species Immunized: Mouse
Purification Method: Protein G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: ELISA, IF, IP
Storage: -20C
Shipped: Cold Packs
Provider
From the laboratory of Edward K. Chan, PhD, University of Florida.
References
  1. Ikeda K, Satoh M, Pauley KM, Fritzler MJ, Reeves WH, Chan EK. Detection of the argonaute protein Ago2 and microRNAs in the RNA induced silencing complex (RISC) using a monoclonal antibody. J Immunol Methods. 2006 Dec 20;317(1-2):38-44.
  2. Hendrickson DG, Hogan DJ, McCullough HL, Myers JW, Herschlag D, Ferrell JE, Brown PO. Concordant regulation of translation and mRNA abundance for hundreds of targets of a human microRNA. PLoS Biol. 2009 Nov;7(11):e1000238.

If you publish research with this product, please let us know so we can cite your paper.

 
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