Anti-Lipovitellin II, Lipoprotein A (LpA) Apoprotein [12H5.A2] Antibody

This mouse IgM monoclonal antibody was generated against lipoprotein/peptides and recognizes Callinectes sapidus (blue crab) Apoprotein [Lipoprotein A (LpA)] fraction of Lipovitellin II (LpII).

Highlights:

  • Recognizes Callinectes sapidus (blue crab) Apoprotein [Lipoprotein A (LpA)] fraction of Lipovitellin II (LpII)
  • Suitable for Immunofluorescence, Immunohistochemistry, Western Blot and ELISA applications

The major component of the egg yolk of most marine invertebrates is lipovitellin. Lipovitellin provides materials and energy for the embryos until they hatch from the egg and begin to feed. Embryos of the blue crab, Callinectes sapidus, develop in egg sacs through a series of stages over 130 days at 28 °C. They emerge from the egg sacs as free swimming zoeae. Until they emerge from the egg sacs, the embryos are nutritionally dependent on lipids and lipoproteins stored as yolk within the eggs. The lipovitellin (LpII) of blue crab egg yolk is an orange colored high density lipoprotein with a hydrated density of 1.16 g/mL, a globular shape, and a sedimentation coefficient of 10.4 Svedberg flotation units. LpII is composed of lipid (48%), protein (50%) and carbohydrate (2%). Phospholipids, primarily phosphatidyl choline, comprise the major lipid class, along with lesser amounts of triacylglycerols and cholesterol, and a non-covalently bound carotenoid that gives lipovitellin a characteristic yellow color. The protein moiety is composed of two apoproteins, referred to as Protein A [107 kilodaltons (kDa)] and Protein B (78 kDa).

From the laboratory of Richard F. Lee, PhD, Skidaway Institute of Oceanography, Department of Marine Sciences at University of Georgia.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EGA209
Anti-Lipovitellin II, Lipoprotein A (LpA) Apoprotein [12H5.A2] Antibody
5mL In stock
Regular Price:$355.00
On Sale:
Specifications

Product Type: Antibody
Antigen: Apoprotein [Lipoprotein A (LpA)] fraction of Lipovitellin II (LpII) from the yolk of Callinectes sapidus egg sacks
Accession ID: P08519
Molecular Weight: 107 kDa
Isotype: IgM
Clonality: Monoclonal
Clone Name: 12H5.A2
Reactivity: Callinectes sapidus (blue crab). Possibly multiple other crustaceans/marine inverterbrates
Immunogen: Lipoprotein/ peptide
Species Immunized: Mouse
Buffer: Cell Culture Supernatant
Tested Applications: ELISA, WB, IF, IHC
Storage: -80C
Shipped: Cold Packs

Provider
From the laboratoty of Richard F. Lee, PhD, Skidaway Institute of Oceanography, Department of Marine Sciences at University of Georgia.
References
  1. Walker A, Ando S, Lee RF. Synthesis of a high-density lipoprotein in the developing blue crab (Callinectes sapidus). Biol Bull. 2003 Feb;204(1):50-6.
  2. Walker A, Lee RF. Lipovitellin and Lipid Droplet Accumulation in Oocytes During Ovarian Maturation in the Blue Crab, Callinectes sapidus. Experimental Zoology. 1995: 271:401-412
  3. Rehn KG, Lee RF. Lipophorin as a lipid carrier in the hemolymph of the horseshoe crab, Limulus polyphemus. Braz J Med Biol Res. 1994 May;27(5):1135-9.

If you publish research with this product, please let us know so we can cite your paper.

Loading...