Anti-Porcine Rotavirus VP6, Serogroup B [10B1] Antibody

This mouse IgG monoclonal antibody was generated against purified recombinant porcine rotavirus (PoRV) VP6 subclass B and is specific for porcine PoRV VP6 subclass B.

Highlights:

  • Recognizes porcine PoRV VP6 subclass B
  • Suitable for Immunofluorescence, Western Blot and ELISA applications

Porcinerotavirus (PoRV) is a non-enveloped icosahedral virus belonging to the genus Rotavirus and family Rotaviridae.  Rotavirus genome has 11 segments which encode 6 non-structure proteins(NSP 1, 2, 3, 4, 5, 6) and 6 structure proteins (VP 1, 2, 3, 4 ,6, 7). Virion has three layers. The outer layer is comprised of VP4 and VP7 which are necessary for virus entry and involved in virus neutralization. The middle layer is VP6 protein,the most abundant and conserve structure protein. Base on the antigenicity and sequence of VP6, rotaviruses are divided into 8 serogroups (A-H). Serogroup A, B, C, E and H have been implicated in swine enteric disease.

From the laboratory of Kyoungjin J. Yoon, DVM, PhD, Iowa State University.

Catalog Number Product Size AVAILABILITY Price Qty
EIS004
Anti-Porcine Rotavirus VP6, Serogroup B [10B1] Antibody, 100ug
100ug 3-5 weeks
Price: $306.00
Specifications
Antigen: Porcine rotavirus (PoRV) VP6
Accession ID: P14162
Molecular Weight: 44.6 kDa
Isotype: IgG
Clonality: Monoclonal
Clone Name: 10B1
Reactivity: Porcine
Immunogen: Purified recombinant PoRV VP6 subclass B
Species Immunized: Mouse
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: ELISA, WB, IF
Storage: -20C
Shipped: Cold Packs
Data

Immunofluorescence Microscopy

Immunofluorescence microscopy of Sf9 cells inoculated with are combinant baculovirus expressing VP6 of PoRV B (A)or PoRV C (B) and baculovirus (C, negative control), respectively, after being stained with mAB10B1 and 11F3. Clones were specific positive to their targets.

Western Blot

Specificity of mAB 10B1 and 11F3 for PoRV B VP6 (A) And PoRV C VP6 (B), respectively as determined by WB

Provider
From the laboratoty of Kyoungjin J. Yoon, DVM, PhD, Iowa State University.
References
  1. Estes, M. K., and A.Z. Kapikian, 2007, Rotaviruses,in D.M.Knipe, and P.M. Howley, eds.,FieldsVirology, 5thEdition, Philadelphia: Lippincott-Raven and Wilkins Publishers,p.1917-1974.
  2. Matthijnssens, J., P. H.Otto, M. Ciarlet, U. Desselberger, M. Van Ranst, and R. Johne, 2012, VP6-sequence-based cut off values as a criterion for rotavirus species demarcation: Arch Virol,v.157,p.1177-82.
  3. Trojnar, E., P. Otto, and R. Johne, 2009, The first complete genome sequence of a chicken group A rotavirus indicates independent evolution of mammalian and avian strains: Virology,v.386,p.325-33.
  4. Cooke, S. J. , I . N . Clarke, R. B. Freitas, Y. B. Gabbay, and P.R. Lambden, 1992, The correct sequence of the porcine group C / Cowden rotavirus major innercapsid protein shows close homology with human isolates from Brazil and the U.K: Virology,v.190,p.531-7.

If you publish research with this product, please let us know so we can cite your paper.

 
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