Anti-Golgi Alpha-Mannosidase 2 Antibody

This rabbit polyclonal antibody was generated against native purified rat Golgi alpha-mannosidase II and recognizes rat and mouse Golgi alpha-mannosidase II.


  • Recognizes rat and mouse Golgi alpha-mannosidase II
  • Does NOT react with human or monkey Golgi alpha-mannosidase II
  • Suitable for Western Blot, Immunoprecipitation, and Immunofluorscence applications

α-mannosidase II is a hydrolase involved in the processing of asparagine-linked oligosaccharides, is an integral membrane glycoprotein facing he lumen of Golgi membranes. The enzyme is also a member of a growing class of Golgi enzymes that contain globular catalytic domains anchored to the lumen of the Golgi complex by a terminal membrane-spanning domain. Man II modifies the GIcNAcMansGlcNAc2 intermediate to complete the mannose trimming reactions and gives rise to the endoglycosidase H (endo H)-resistant oligosaccharide, GIcNAcMan3GlcNAc2.

From the laboratory of Kelley W. Moremen, PhD, University of Georgia.

Catalog Number Product Size AVAILABILITY Price Qty
Anti-Golgi Alpha-Mannosidase 2 Antibody, 50uL
50uL In stock
Price: $306.00
Product Type: Antibody
Antigen: Rat Golgi alpha-mannosidase II
Accession ID: NCBI RefSeq: NP_037111, Uniprot P28494
Molecular Weight: ~110 kDa
Clonality: Polyclonal
Reactivity: Rat, human and possibly others
Immunogen: Purified protein
Species Immunized: Rabbit
Buffer: Rabbit Serum
Tested Applications: IF
Storage: -80C
Shipped: Dry Ice


Immunofluorescence localization of Golgi Man II in NRK cells showing that these enzymes are concentrated in the Golgi region. Cells were grown on coverslips, fixed in paraformaldehyde, permeabilized and processed for immunofluorescence

Adapted from: Velasco A et al., J Cell Biol. 1993 Jul;122(1):39-51.
From the laboratory of Kelley W. Moremen, PhD, University of Georgia.
  1. Moremen KW, Touster O, Robbins PW. Novel purification of the catalytic domain of Golgi alpha-mannosidase II. Characterization and comparison with the intact enzyme. J Biol Chem. 1991 Sep 5;266(25):16876-85.
  2. Velasco A, Hendricks L, Moremen KW, Tulsiani DR, Touster O, Farquhar MG. Cell type-dependent variations in the subcellular distribution of alpha-mannosidase I and II. J Cell Biol. 1993 Jul;122(1):39-51.

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